Electrophoresis

Elektrophorese
Electrophoresis · Gel Staining
Fluorescent Staining of
Nucleic Acids
Ethidium bromide-free staining
New, non-toxic and non-carcinogenic staining dye for
fluorescent staining of nucleic acids in agarose gels.
Highly sensitive and applied just like ethidium bromide.
Resulting brightly-green signals may be detected by
standard ethidium bromide photo filters.
Roti ® -Safe GelStain
ready-to-use, for electrophoresis
For fluorescent staining of nucleic acids in agarose gels.
Store at +4 °C
Roti ® -Safe GelStain is a new, versatile staining reagent for
detection of all nucleic acids in agarose gels.
Roti ® -Safe GelStain detects up to 1.5 ng/mm 2 nucleic acid, which is
approx. 0.2 ng per band, and is, therefore, as sensitive as ethidium
bromide. The dye may be used exactly in the same way as ethidium
bromide - for staining in-gel, staining through running buffer, or post-run
staining -, and may replace this toxic and potentially mutagen reagent
completely.
When bound to nucleic acids in agarose gels, Roti ® -Safe GelStain emits a
brightly green fluorescence that may be documented by standard ethidium
bromide foto filters. Roti ® -Safe GelStain is compatible with all standard
down-stream applications and has been successfully tested for use with
gel extraction, ligation, transformation and transfection.
Excitation maximum (bound to DNA): 290-320 nm
Emission maximum (bound to DNA): 515 nm
Suitable for staining of dsDNA, ssDNA and RNA. 1 ml is sufficient
for staining of approx. 600 minigels (with 30 ml agarose each).
3865.1 1 ml plastic 64,85 €
3865.2 5 x 1 ml plastic 297,15 €
Please note our Technical Information Brochures on the Internet
(Life Science Main Page):
Gel Staining of Agarose Gels
Ethidiumbromide, methylene blue and Roti ® -Safe GelStain
Roti ® -Safe GelStain
Basics and Safety data
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Global Step - Email : Office@global-step.net
Telefon : (+4) 0742 095 539
Tel/Fax : (+4) 031 104 85 87
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Ethidium bromide solutions
In spite of newly developed fluorescent staining
reagents ethidium bromide remains the standard
staining technic for nucleic acid in electrophoresis.
Ethidium bromide is a carcinogenic substance which
shouldn't come in contact with skin.
In order to minimise this danger we offer ready-to-use
ethidium bromide solution in dropper bottles for easy
handling and maximum safety.
Excitation maxima (unbound): 210 nm, 285 nm
Excitation maximum (bound to DNA): 366 nm
Excitation possible via DNA at 254 nm
Emission maximum: 605 nm.
Ethidium bromide solution 0.025 % in dropper bottle
aqueous solution (250 µg/ml)
For staining of DNA or RNA in agarose gels during electrophoresis.
i Danger H331
Application:
Two drops will suffice for staining a 50 ml agarose gel.
Add directly to melted agarose.
Please store in the dark!
HP47.1 15 ml plastic 24,70 €
Ethidium bromide solution 0.5 % in dropper bottle
aqueous solution (5 mg/ml)
For counterstaining of nuclei in histology and cytology.
For staining of DNA/RNA after electrophoresis.
g Warning H332
Application:
In order to prepare a staining solution add 5 drops of ethidium bromide
solution to 500 ml water, buffer, or electrophoresis running buffer,
respectively.
Nuclei / nucleic acids are stained red.
Please store in the dark!
HP46.1 15 ml plastic 24,70 €
Safety data sheets
can be found on the Internet next to the product description.
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