Electrophoresis

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<strong>Electrophoresis</strong> · Markers Roti ® -Mark 10-150 PLUS ➂ Protein marker with additional prestained protein Size range: 10-150 kD Number of fragments: 9 Number of lanes/ml: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. Reliable size determination as well as control of running behaviour and transfer. This marker represents an advanced version of marker Roti ® -Mark 10-150 and is supplemented with an additional prestained carbo anhydrase (approx. 27 kD). Roti ® -Mark 10-150 PLUS consists of a set of genetically engineered proteins with defined, regular molecular weight. The proteins are not glycosylated and therefore display homogeneous electrophoretic behaviour. Whilst in prestained markers the apparent molecular size in the gel may differ from the actual size of the proteins, Roti ® -Mark 10-150 PLUS exhibits a strictly size-dependent running behaviour. The additional prestained protein enables easy control of the run as well as of the subsequent transfer. Therefore, this marker adds the benefits of prestained protein markers to a reliable determination of the molecular weight of separated proteins. The enhanced 40 kD-band allows easy orientation in the gel. Roti ® -Mark 10-150 has been assembled from a set of His-tagged, recombinant proteins that may be detected during Western-Blot analysis using anti-His antibodies. Fig.: 4-20 % gradient SDS-PAGE (Tris) X879.2 100 μl 42,95 € X879.1 500 μl 109,65 € Roti ® -Mark PRESTAINED ➃ Protein marker, prestained Size range: 17-245 kD Number of fragments: 7 Number of lanes/ml: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. The proteins of this marker are prestained. Roti ® -Mark PRESTAINED is, therefore, an ideal size marker for controlling the gel run and for quick and reliable evaluation of transfer efficiency during Western Blot. Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of our unstained marker Roti ® -Mark 10-150 PLUS (Art. No. X879) with one prestained protein. Fig.: 12 % SDS-PAGE (Tris) T852.3 100 μl 41,95 € T852.1 250 μl 71,20 € T852.2 4 x 250 μl 264,45 € Roti ® -Mark BICOLOR ➄ re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use TIP re TTTIIPP re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use Protein marker, prestained Size range: 17-245 kD Number of fragments: 9 Number of lanes/ml: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. New marker including bicolour-prestained proteins of 17 up to 245 kD. In order to enhance conspicuity of separation during the gel run and for better orientation in the gel, two proteins of approx. 25 and 55 kD have been prestained in red in addition to the blue coloured bands. All proteins excel by having a very even running behaviour and form distinct bands. The marker is designed as ready-to-use solution for direct application to the gel. Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of our unstained marker Roti ® -Mark 10-150 PLUS (Art. No. X879) with one prestained protein. Fig.: 4-20 % gradient SDS-PAGE (Tris) 2806.1 100 μl 45,70 € 2806.2 250 μl 83,35 € Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 Roti ® -Mark WESTERN Marker ➅ Protein marker, not prestained Size range: 10-120 kD Number of fragments: 8 Number of lanes/ml: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. Finely graded protein marker for medium size range. The unstained proteins enable correct size evaluation in gel. The marker-specific antibody Roti ® -Mark WESTERN HRP-conjugate (Art. No. 0949.1) enables the marker to be detected directly in Western-Blot and stained through chemoluminescence or colour reaction. Consequently you will obtain the ladder size directly on your printout, X-ray film or membrane - this rules out the possibility of any errors through inaccurate transfer of the bands. Marker proteins are solubilised in a non-reducing buffer acc. to Lämmli. Fig.: 12 % SDS-PAGE (Tris), 10 µl and 5 µl marker solution, respectively. 0948.1 500 μl 131,15 € Roti ® -Mark WESTERN Set ➆ Set of protein marker and antibody for gel and Western-Blot Size range: 10-120 kD Number of fragments: 8 Number of lanes: approx. 100 or 10 (mini gels) Store at -20 °C Transport temp.: room temp. Finely graded protein marker for medium size range and for detection in gel and on the Western-Blot membrane. The marker itself consists of unstained proteins and therefore enables correct size evaluation in gel (left figure). The marker-specific antibody Roti ® -Mark WESTERN HRP- conjugate (rabbit) enables the marker to be detected directly in Western- Blot and stained through chemoluminescence or colour reaction. Antibody incubation is performed in parallel with the incubation of your protein- specific antibodies and therefore saves any additional work and expense. The marker bands are also automatically stained (right figure) during subsequent detection by horseradish-peroxidase. Consequently you will obtain the correct ladder size directly on your printout, X-ray film or membrane, ruling out the possibility of any errors through inaccurate transfer of the bands. Marker proteins are solubilised in a non-reducing buffer acc. to Lämmli. Roti ® -Mark WESTERN Marker can be bought seperately under Art. No. 0948.1 and Roti ® -Mark WESTERN HRP conjugate under Art. No. 0949.1. Left hand fig.: 12 % minigel, 10 µl and 5 µl marker solution, respectively. Right hand fig.: Chemoluminescence detection via Roti-Lumin, Art. No. P078.1. Proteins were run in a 12 % minigel and transferred onto a Roti ® -PVDF-Membran. 5 µl Roti ® -Mark WESTERN Marker and Roti ® -Mark WESTERN HRP conjugate diluted by 1:2.500. 0947.2 1 Mini set 30,65 € 0947.1 1 set 244,05 € Instructions for use can be found on the Internet next to the product description. re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use TIP re TTTIIPP re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use 863 Elektrophorese
Elektrophorese <strong>Electrophoresis</strong> · Markers DNA-Markers Roth offers a multifaceted marker range including DNA markers for each application required. Our DNA-markers produce sharp, clear bands. Terminally, the unregular markers carry sticky Eco RI sites and, therefore, end-labelling methods can be applied easily. The DNA-markers are supplied in lyophilised form. Gel loading buffer for dissolving the lyophilised DNA-fragments is included. Roti ® -Load DNA with glycerol (Art. No. 0100.1), or the specially designed short-run-loading buffer (Art. No. 0099.1) is used as a gel loading buffer. Unevenly Distributed DNA-Markers (suitable for DNA-quantitation in the gel) Marker pUC19-Marker pUC19/MspI pBR328-Marker pBR328 Mix I Lambda Hind III / phiX Hae III Marker Lambda Hind III Marker ready-to-use ready-to-use Art. No. X901 (fig. 1) T149 (fig. 2) X902 (fig. 3) T146 (fig. 4) CP49 (fig. 5) X910 (fig. 6) Size range (bp) 26 - 501 26 - 501 154 - 2176 154 - 2176 72 - 23130 125 - 23130 Number of fragments 10 10 11 11 17 8 Reinforced bands at (bp) - - - - - - Number of lanes in minigel 200 / ml 200 / 100 μg 200 / ml 200 / 100 μg 200 / 100 μg 200 / 100 μg Delivery ready-to-use lyophilized ready-to-use lyophilized lyophilized lyophilized DNA-marker ready-to-use Size range: 26-501 bp Number of fragments: 10 Number of lanes per ml: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. The marker was manufactured using pUC19-DNA and restriction endonuclease Msp I. Made from natural plasmids: fragment sizes can be validated! DNA-ladder for analysis of small DNA fragments. The concentration of the individual fragments is equimolar so that rough quantification of the analysed DNA is possible. The ready-to-use pUC19-marker has already been dissolved in 1x concentrated gel loading buffer Roti ® -Load DNA (with glycerol, Art. No. 0100.1) and can be used directly. It has a concentration of 0.1 µg DNA/µl marker-solution. Fig.: 1 % agarose (NEEO, Art. No. 2267) + 2 % SynergelTM (Art. No. 0184) 864 (bp) - 501/489 - 404 - 331 - 242 - 190 - 147 - 111/110 - 67 - 34 - 26 pUC19-Marker ready-to-use ➀ (bp) - 2176 - 1766 - 1230 - 1030 - 653 - 517 - 453 - 394 - 298 - 234/220 - 154 X901.1 500 μl 48,85 € X901.2 4 x 500 μl 173,20 € (bp) - 23130 - 9416 - 6557 - 4361 - 2322 - 2027 - 1353 - 1078 - 872 - 603/564 - 310 - 281/271 - 234 - 194 - 72 ➀+➁ ➂+➃ ➄ ➅ re re re re re re re read ad ad ad ad ad adyyyyyyyy to to to to to to to to-u -u -u -u -u -uuu se se se se se se se see yy readyto-use pUC19/Msp I ➁ Global Step - Email : Office@global-step.net Telefon : (+4) 0742 095 539 Tel/Fax : (+4) 031 104 85 87 Mobil : (+4) 0720 095 539, 0723 442 593 (bp) - 23130 - 9416 - 6557 - 4361 - 2322 - 2027 - 564 DNA-marker + gel loading buffer Size range: 26-501 bp Number of fragments: 10 Number of lanes per 100 µg: approx. 200 (mini gels) Store at -20 °C Transport temp.: room temp. The marker was manufactured by using pUC19-DNA and restriction endonuclease Msp I. Made from natural plasmids: fragment sizes can be validated! DNA-ladder for analysis of small DNA fragments. The concentration of the individual fragments is equimolar so that rough quantification of the analysed DNA is possible. Fig.: 1 % agarose (NEEO, Art. No. 2267) + 1 % Synergel TM (Art. No. 0184) The DNA marker is supplied in lyophilised form. Sample buffer for dissolving the lyophilised DNA-fragments is included. T149.1 50 μg + gel loading buffer 58,90 € T149.2 4 x 50 μg + gel loading buffer 184,50 €
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Electrophoresis

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