Electrophoresis

Electrophoresis · Gel Staining
Rotiphorese ® Blue R
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Brillant Blue R concentrate
For Coomassie staining of protein gels.
l n i Danger H225-H370-H315-H319-H301-H311-H331
Concentrate for easy preparation of Coomassie Blue R staining solutions.
Rotiphorese ® -blue is ideal for staining proteins in SDS-PAGE gels. It saves
any tedious weighing of staining powder and having to prepare the mixture.
Figure:
Gel stained by Rotiphorese ® -Blue R, 12 % PAA in Tris,
Roti ® -Mark STANDARD (Art. No. T851).
Concentrate contains Coomassie Blue R, methanol and pure acetic acid.
Rotiphorese ® concentrate Blue R can be diluted with 400-600 ml deionised
water to obtain the following compositions:
Dilution with water 397.5 ml 507.5 ml 602.5 ml
yields staining solution approx. 1 l approx. 1.1 l approx. 1.2 l
methanol 50 % 45 % 41.5 %
pure acetic acid 10 % 9 % 8.3 %
water 39.75 % 45.78 % 50 %
Blue R 0.25 % 0.22 % 0.2 %
Dilution can be carried out in the supplied bottle.
The solution should be filtered before use.
3074.1 600 ml glass 25,65 €
Brilliant Blue R 250 (C.I. 42660)
for preparing staining solution for the protein analysis in gel
electrophoresis
Coomassie Brillant Blue R 250
C 45 H 44 N 3 NaO 7 S 2
M 825,99 g/mol
Only conditionally use for staining of membranes after protein transfer.
3862.1 10 g glass 18,10 €
3862.2 100 g glass 71,80 €
• White light-light boxes see page 559
Safety data sheets
can be found on the Internet next to the product description.
Roti ® -Black P
silver staining kit for proteins
o h i n j Danger
H272-H301-H311-H331-H314-H317-H351-H410
• Sensitive silver staining
• Transparent gel
• Reliable results
Roti ® -Black is a kit for speedy, reproducible silver staining of proteins in
free and foil-supported gels.
High sensitivity in combination with very low background enables detection
of proteins from 1 ng/mm 2 on. 1 kit is adequate for staining 10 gels. Staining
can be performed in unsupported gels without methanol. Included is an
easy-to-follow instruction manual which will guarantee reliable results.
L533.1 for 10 gels of 10 ml vol. each 59,80 €
L533.2 for 10 gels of 40 ml vol. each 75,90 €
Silver staining of proteins and DNA-gels
Roti ® -Black silver staining kits have been optimized to provide you with the most
sensitive staining possible. Adding thiosulfate during the sensibilization and
generation process reduces the background and increases contrast (acc. to Blum et al.,
(1987) Electrophoresis 8:93-9). Formaldehyde should preferably be fresh when
applied and it reduces the silver ions (Ag + ) to so-called silver seed crystals.
We recommend to:
• Wash the gels carefully after each step otherwise there may be side reactions in
the form of stains, spots, increased background or secondary bands.
• Fix the gels carefully. Inadequate fixation impairs band formation and increases
the background. Fresh and high-quality fixatives should be used and adapted to
the volume and duration of gel strength.
Please note our Technical Information Brochure on the Internet
(Life Science Main Page):
Coomassie Gel Staining
Basics and mechanisms of Coomassie dyes
Global Step - Email : Office@global-step.net
Telefon : (+4) 0742 095 539
Tel/Fax : (+4) 031 104 85 87
Mobil : (+4) 0720 095 539, 0723 442 593
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