Electrophoresis

Elektrophorese
Electrophoresis · PAGE Solutions
Acrylamide and bisacrylamide
stock solutions:
Rotiphorese ® Gel A and B
The pore size of acrylamide gels may be altered by varying the total gel
concentration (% T) and the percentage of the crosslink (% C). Gels with
every desired T/C rate can be produced with Rotiphorese ® Gel A and B.
Required formulas:
V a = Volume of Gel A in ml
V b = Volume of Gel B in ml
V t = Total volume of gel casting solution (ml)
%T = gel concentration in % = % Acrylamide + Bisacrylamide
%C = % crosslinking = (% Bisacrylamid x 100) / T
V a = (T x (100-C) x V t ) / 3000
V b = (T x C x V t) / 200
Example:
To make 100 ml gel with 10 % T and 2.7 % C, calculate as follows:
V a = (10 x (100-2.7) x 100) / 3000 = 32.43 ml Gel A
V b = (10 x 2.7 x 100) / 200 = 13.5 ml Gel B
Combine 32.43 ml Gel A and 13.5 ml Gel B, fill up the volume to 100 ml with
your usual buffer, deaerate, then mix with APS and TEMED. Cast the gel.
Rotiphorese ® Gel A
30 % Acrylamide solution
Store at +8 °C
n g Danger H340-H350-H372-H361f-H302-H312-H315-H319-H317
Ready-to-use, stabilised 30 % acrylamide stock solution.
3037.1 1 l glass 43,25 €
Original pack 6 x 1 l 41,09/1 l
Rotiphorese ® Gel B
2 % Bisacrylamide solution
Store at +8 °C
Ready-to-use, stabilised 2 % acrylamide stock solution.
3039.2 250 ml glass 15,65 €
3039.1 1 l glass 38,55 €
Original pack 6 x 1 l 36,62/1 l
850
Rotiphorese ® NF ready-to-use
solutions for automatic
sequencing
Rotiphorese ® ready-to-use solutions for automatic
sequencing are special fluorescence-free acrylamide/
bisacrylamide mixtures of highest quality as required for
automatic sequencing.
Made from 4x cristallised acrylamide.
Rotiphorese ® NF-Acrylamide/
Bis-solution 30 % (29:1)
ready-to-use, gas stabilised, aqueous 30 % acrylamide and
bisacrylamide stock solution at a ratio of (29:1), fluorescence free
Tested for automatic sequencers.
Store at +8 °C
n g Danger H340-H350-H372-H361f-H302-H312-H315-H319-H317
A124.1 250 ml glass 38,90 €
A124.2 1 l glass 116,70 €
Original pack 6 x 1 l 110,87/1 l
Rotiphorese ® NF-Acrylamide/
Bis-solution 40 % (19:1)
ready-to-use, gas stabilised, aqueous 40 % acrylamide and
bisacrylamide stock solution at a ratio of (19:1), fluorescence free
Tested for automatic sequencers.
Store at +8 °C
n g Danger H340-H350-H372-H361f-H302-H312-H315-H319-H317
A516.1 250 ml glass 39,90 €
Rotiphorese ® NF-Acrylamide/
Bis-solution 40 % (29:1)
ready-to-use, gas stabilised, aqueous 40 % acrylamide and
bisacrylamide stock solution at a ratio of (29:1), fluorescence free
Tested for automatic sequencers.
Store at +8 °C
n g Danger H340-H350-H372-H361f-H302-H312-H315-H319-H317
A121.1 250 ml glass 32,85 €
• Fluorescence free urea see page 852
• Fluorescence free 10 x TBE buffer see page 859
Fluorescence-free
NF acrylamide solutions are very well suited for application in fluorescence-labelled
automated sequencing, e.g. using LI-COR or ABI slab gel sequencers.
Please note our Technical Information Brochure
on the Internet (Life Science Main Page):
Gels made of Polyacrylamide
General technical informations
Global Step - Email : Office@global-step.net
Telefon : (+4) 0742 095 539
Tel/Fax : (+4) 031 104 85 87
Mobil : (+4) 0720 095 539, 0723 442 593
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