Electrophoresis

Electrophoresis · Markers
Roti ® -Mark 10-150 PLUS ➂
Protein marker with additional prestained protein
Size range: 10-150 kD
Number of fragments: 9
Number of lanes/ml: approx. 200 (mini gels)
Store at -20 °C
Transport temp.: room temp.
Reliable size determination as well as control of running behaviour
and transfer.
This marker represents an advanced version of marker Roti ® -Mark
10-150 and is supplemented with an additional prestained carbo anhydrase
(approx. 27 kD). Roti ® -Mark 10-150 PLUS consists of a set of genetically
engineered proteins with defined, regular molecular weight. The proteins
are not glycosylated and therefore display homogeneous electrophoretic
behaviour. Whilst in prestained markers the apparent molecular size in the
gel may differ from the actual size of the proteins, Roti ® -Mark 10-150 PLUS
exhibits a strictly size-dependent running behaviour. The additional prestained
protein enables easy control of the run as well as of the subsequent
transfer. Therefore, this marker adds the benefits of prestained protein
markers to a reliable determination of the molecular weight of separated
proteins. The enhanced 40 kD-band allows easy orientation in the gel.
Roti ® -Mark 10-150 has been assembled from a set of His-tagged,
recombinant proteins that may be detected during Western-Blot analysis
using anti-His antibodies.
Fig.: 4-20 % gradient SDS-PAGE (Tris)
X879.2 100 μl 42,95 €
X879.1 500 μl 109,65 €
Roti ® -Mark PRESTAINED ➃
Protein marker, prestained
Size range: 17-245 kD
Number of fragments: 7
Number of lanes/ml: approx. 200 (mini gels)
Store at -20 °C
Transport temp.: room temp.
The proteins of this marker are prestained. Roti ® -Mark PRESTAINED is,
therefore, an ideal size marker for controlling the gel run and for quick and
reliable evaluation of transfer efficiency during Western Blot.
Please note: Reliable size determination of proteins cannot be performed
using prestained markers. For this, we recommend the use of
our unstained marker Roti ® -Mark 10-150 PLUS (Art. No. X879) with
one prestained protein.
Fig.: 12 % SDS-PAGE (Tris)
T852.3 100 μl 41,95 €
T852.1 250 μl 71,20 €
T852.2 4 x 250 μl 264,45 €
Roti ® -Mark BICOLOR ➄
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Protein marker, prestained
Size range: 17-245 kD
Number of fragments: 9
Number of lanes/ml: approx. 200 (mini gels)
Store at -20 °C
Transport temp.: room temp.
New marker including bicolour-prestained proteins of 17 up to 245 kD. In
order to enhance conspicuity of separation during the gel run and for better
orientation in the gel, two proteins of approx. 25 and 55 kD have been prestained
in red in addition to the blue coloured bands. All proteins excel by
having a very even running behaviour and form distinct bands. The marker
is designed as ready-to-use solution for direct application to the gel.
Please note: Reliable size determination of proteins cannot be performed
using prestained markers. For this, we recommend the use of
our unstained marker Roti ® -Mark 10-150 PLUS (Art. No. X879) with
one prestained protein.
Fig.: 4-20 % gradient SDS-PAGE (Tris)
2806.1 100 μl 45,70 €
2806.2 250 μl 83,35 €
Global Step - Email : Office@global-step.net
Telefon : (+4) 0742 095 539
Tel/Fax : (+4) 031 104 85 87
Mobil : (+4) 0720 095 539, 0723 442 593
Roti ® -Mark WESTERN Marker ➅
Protein marker, not prestained
Size range: 10-120 kD
Number of fragments: 8
Number of lanes/ml: approx. 200 (mini gels)
Store at -20 °C
Transport temp.: room temp.
Finely graded protein marker for medium size range. The unstained
proteins enable correct size evaluation in gel. The marker-specific antibody
Roti ® -Mark WESTERN HRP-conjugate (Art. No. 0949.1) enables the marker
to be detected directly in Western-Blot and stained through chemoluminescence
or colour reaction. Consequently you will obtain the ladder
size directly on your printout, X-ray film or membrane - this rules out
the possibility of any errors through inaccurate transfer of the bands.
Marker proteins are solubilised in a non-reducing buffer acc. to Lämmli.
Fig.: 12 % SDS-PAGE (Tris), 10 µl and 5 µl marker solution, respectively.
0948.1 500 μl 131,15 €
Roti ® -Mark WESTERN Set ➆
Set of protein marker and antibody for
gel and Western-Blot
Size range: 10-120 kD
Number of fragments: 8
Number of lanes: approx. 100 or 10 (mini gels)
Store at -20 °C
Transport temp.: room temp.
Finely graded protein marker for medium size range and for detection in gel
and on the Western-Blot membrane. The marker itself consists of unstained
proteins and therefore enables correct size evaluation in gel (left figure).
The marker-specific antibody Roti ® -Mark WESTERN HRP- conjugate
(rabbit) enables the marker to be detected directly in Western- Blot and
stained through chemoluminescence or colour reaction. Antibody incubation
is performed in parallel with the incubation of your protein- specific
antibodies and therefore saves any additional work and expense. The marker
bands are also automatically stained (right figure) during subsequent
detection by horseradish-peroxidase. Consequently you will obtain the correct
ladder size directly on your printout, X-ray film or membrane, ruling out
the possibility of any errors through inaccurate transfer of the bands. Marker
proteins are solubilised in a non-reducing buffer acc. to Lämmli. Roti ® -Mark
WESTERN Marker can be bought seperately under Art. No. 0948.1 and
Roti ® -Mark WESTERN HRP conjugate under Art. No. 0949.1.
Left hand fig.: 12 % minigel, 10 µl and 5 µl marker solution, respectively.
Right hand fig.: Chemoluminescence detection via Roti-Lumin,
Art. No. P078.1. Proteins were run in a 12 % minigel and transferred onto a
Roti ® -PVDF-Membran. 5 µl Roti ® -Mark WESTERN Marker and Roti ® -Mark
WESTERN HRP conjugate diluted by 1:2.500.
0947.2 1 Mini set 30,65 €
0947.1 1 set 244,05 €
Instructions for use
can be found on the Internet next to the product description.
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Elektrophorese