THESIS

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the investigations revealed that the failure of seed set in hybrids was due to the ovule abortion. In order to overcome the sterility, the colchicine has been applied to the interspecific hybrids. The pollen fertility of S. americanum x S. torvum (59.34 %) (Table 21) was lower than their parent but not too much different. This result could not confirm that the hybrids from the crosses between S. americanum x S. torvum were true hybrids or not; we have to confirm with poildy levels determination and also the morphological characters. The hybrids wihin S. americanum x S. americnum gave lower pollen fertility than their parents. Similar results have been obtained for F1 hybrids from the same species combination (Rajasekaran, 1970; Rangasamy and Kadambavanasundaram, 1974). The low pollen fertility of the F1 may be attributed to meiotic difficulty arising from hybridity of the nucleus (Isshiki and Kawajiri, 2002). Pollen of F1 hybrids between S. americanum x S. villosum was sterile (~ 0.00 %) (Table 21). The variations in the level of low pollen fertility could be due to either the differences in genetic constitution or cryptic structural differences between the parent chromosomes, which might be too small to be detected cytologically or due to combined effects of both factors (Rao, 1981; Anis et al., 1994). Moreover, the hybrids of S00859 x S00860 and its reciprocal between S. americanum x S. villosum had very high pollen fertility (Table 21), this could be due to both of accessions were very closely related but the ploidy levels determination and the morphological characters have yet to be confirmed. In cantrast, pollen fertility of the interspecific hybrids between S. americanum x S. nigrum and S. villosum x S. nigrum were insignificantly lower than their parents. This also has to confirm the interspecific hybrids by polidy levels determination and the morphological characters. 99
However, pollen fertility of the intraspecific hybrids of S. villosum is high (Table 21). This result shows that the accessions within same species closely related genetic affinity produced fertile hybrids with regular chromosome pairing, while the hybrids of the distantly related species have meiotic irregularities and are sterile (Marfil et al., 2006). 100
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THESIS GENETIC DIVERSITY IN THE SOU
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ACKNOWLEDGEMENTS I would like to ex
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LIST OF TABLES Table Page 1 Ninety
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LIST OF FIGURES Figure Page 1 Selec
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LIST OF FIGURES (Continued) Figure
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LIST OF FIGURES (Continued) Appendi
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LIST OF ABBREVIATIONS cm = centimet
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Asian Vegetable Research and Develo
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OBJECTIVES 1. To characterize using
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campanulate, rotate or copular, mos
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Moreover, genetic diversity has an
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Therefore, germplasm characterizati
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some degree from each other and the
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Interspecific hybrids between wild
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Moreover, lines of S. melongena sho
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(qualitative) traits. The cluster a
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Plots of the clustering statistics
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Table 1 (Continued) Temporary No. S
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2. Genetic relationships of Solanum
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pollen grains from randomly selecte
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RESULTS AND DISCUSSION In this stud
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Furthermore, TS02950 collected in C
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distinct from those species. Fruit
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yellow and globose. While, TS001447
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Table 4 Identification of 43 access
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Table 5 Re-identification of 5 acce
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accessions, hence, considered to ha
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Figure 1 Selected items from PROC U
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Table 7 Correlation coefficients sc
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N a m e o f V a r i a b l e o r C l
Page 63 and 64: 1.2.2 Cluster analysis I. Ratio sca
Page 65 and 66: P S E U D O F S T A T I S T I C Sto
Page 67 and 68: C U B I C C L U S T E R I N G C R I
Page 69 and 70: Table 9 Accessions number of Solanu
Page 71 and 72: Table 9 (Continued) Cluster Subclus
Page 73 and 74: Stratifying the accessions into 10
Page 75 and 76: (single fruit). It has also the wid
Page 77 and 78: were collected in Laos, while S. ma
Page 79 and 80: II. Nominal traits (qualitative tra
Page 81 and 82: Table 11 (Continued) Nominal traits
Page 83 and 84: The similarity coefficient values (
Page 85 and 86: (3 accessions). Subclaster A is cha
Page 87 and 88: 8 6 4 Figure 7 Cluster analysis of
Page 89 and 90: Table 12 (Continued) Cluster Subclu
Page 91 and 92: However, the determination of the m
Page 93 and 94: This result is in agreement with th
Page 95 and 96: herbicide (atrazine) resistant trai
Page 97 and 98: 3) Cross - compatibility among S. a
Page 99 and 100: when the larger-flowered species we
Page 101 and 102: Table 14 Interspecific crossability
Page 103 and 104: Table 15 Interspecific crossability
Page 105 and 106: Table 17 Intra- and interspecific c
Page 107 and 108: Table 18 (Continued) Cross combinat
Page 109 and 110: A B C D E F G H Figure 8 The hybrid
Page 111 and 112: Figure 10 F1 hybrid plant from S. m
Page 113: Confirmation of interspecific hybri
Page 117 and 118: Table 19 The pollen fertility of F1
Page 119 and 120: Table 21 (Continued) Species Access
Page 121 and 122: S00860) showed a same ploidy level
Page 123 and 124: Moreover, an accurate determination
Page 125 and 126: S. americanum (S00269) (2n) S. amer
Page 127 and 128: S. villosum (S00854) (4n) S. villos
Page 129 and 130: S. villosum (S00860) (4n) S. villos
Page 131 and 132: Moreover, the crosses between S. am
Page 133 and 134: S. melongena (S00625) S. melongena
Page 135 and 136: S. melongena (S00809) F1 S. villosu
Page 137 and 138: S. americanum (S00861) S. americanu
Page 139 and 140: S. americanum (S00269) S. americanu
Page 141 and 142: S. villosum (TS02600) F1 S. villosu
Page 143 and 144: S. villosum (S00854) F1 S. american
Page 145 and 146: S. nigrum (TS02930) F1 S. americanu
Page 147 and 148: 132 For the interspecific crossing
Page 149 and 150: S. nigrum). For the interspecific c
Page 151 and 152: , and M. Ahmand.1989. Morphological
Page 153 and 154: 138 Collonnier, C., I. Fock, V. Kas
Page 155 and 156: and J.A. Chweya. 1997. Black Nights
Page 157 and 158: and . 1988b. Organelle composition
Page 159 and 160: Laban, J.C.G. 2003. Genetic Diversi
Page 161 and 162: spinous Solanums. Theoret. Appl. Ge
Page 163 and 164: Singh, J. and M. Rai. 2005. Eggplan
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APPENDICES 150
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SEEDLING DATA AVRDC-GRSU CHARACTERI
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S300 Leaf blade tip angle 1 = Very
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S530 Fruit length/breadth ratio 1 =
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S640 Fruit calyx length (N=10) ____
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Appendix Table A1 Characterization
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Appendix Table A1 (Continued) Tempo
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Appendix Table A4 List of morpholog
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Appendix Figure A2 Morphological ch
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Appendix Figure A8 Morphological ch
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Appendix B Identification of specie
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Moments N 91 Sum Weights 91 Mean 7.
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Moment N 91 Sum Weights 91 Mean 101
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Moments N 91 Sum Weights 91 Mean 10
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THESIS

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