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132<br />

For the interspecific crossing among S. melongena and other three species (S.<br />

americanum, S. villosum and S. nigrum, S. villosum is more closely related to S.<br />

melonegena than S. americanum and S. nigrum. This due to the crosses between S.<br />

melongena and those three species gave hybrid plants but when confirmed the<br />

hybridity by pollen fertility, ploidy levels and morphological characters, they were not<br />

the true hybrid. In contrast, when used S. melongena as male parent, the hybrid fruits<br />

were obtained but no viable seeds from the cross between S. melongena and S.<br />

villosum.<br />

Moreover, from the interspecific hybridization among S. torvum and the three<br />

species (S. americanum, S. villosum and S. nigrum), S. villosum is more closely<br />

related to S. torvum than S. americanum and S. nigrum when considered the<br />

percentages of fruit setting and number of seeds per fruit. S. villosum has the higest<br />

percentages of fruit setting and number of seeds per fruit when compare to S.<br />

americanum and S. nigrum (Table 17).<br />

Furthermore, from the interspecific hybridization among S. americanum, S.<br />

villosum and S. nigrum, we found that S. americanum is more closely related to S.<br />

villosum than S. nigrum when considered the percentages of fruit setting and number<br />

of seeds per fruit (Table 18).<br />

Therefore, germplasm characterization provides essential, valuable<br />

information for breeding programs. Knowledge on genetic diversity and relationships<br />

among the S. melongena germplasm may play significant role in breeding programs to<br />

improve fruit quality and resistance to biotic and abiotic stresses of S. melongena.<br />

Interspecific hybridization is possible between Solanum spp. This may accelerate<br />

diversity and increase gene pool for breeding programs. Diversity within germplasm<br />

is critical for S. melongena breeding programs. However, all F1 hybrids from<br />

interspecific hybridization are sterile which may be overcome by using hormone<br />

and/or some chemical such as GA (gibberlleic acid) and colchicines for doubling the<br />

set of chromosome.

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