THESIS
THESIS
THESIS
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1.2.2 Cluster analysis<br />
I. Ratio scale traits (quantitative traits)<br />
The 69 accessions belonging to Solanum spp. was analysed based on<br />
the 13 traits selected using the PROC CLUSTER statement. This procedure searched<br />
throughout the data for accessions that were similar enough to be considered as part of<br />
a common cluster (Cababasay, 1996).<br />
Using the standardized data, the Unweighted Pair Group of<br />
Matheretic Average (UPGMA) was chosen to stratify the 69 accessions. This could be<br />
attributed to the fact that average linkage method (UPGMA) can be used with any<br />
resemblance coefficients compared with Ward’s method. Moreover, the similarity<br />
between pairs of clusters can be determined in a manner less extreme compare with<br />
single or complete linkage (Romesburg, 1984). This was done by taking the average<br />
distance between all pairwise combinations of observations one in each cluster (SAS,<br />
1999). This method was also recommended by Cababasay (1991) due to its best overall<br />
performance.<br />
The PROC CLUSTER statement provided useful statistics (pseudo<br />
F, pseudo t 2 and cubic clustering criteria) that can estimate the number of clusters that<br />
will be formed out of a given data. They refer to the values that are measures of fit for<br />
the analysis of each specified number of clusters (Callantes, 2003). These statistics<br />
were given using the PSEUDO and CCC options, respectively, which are one of the<br />
options used to control the printing of the cluster history (SAS, 1999).<br />
With pseudo F statistic, Figure 3 reveals that the possible numbers<br />
of clusters used to stratify the 69 accessions of Solanum spp. were 2 and 10. With the<br />
pseudo t 2 statistic, however, Figure 4 shows that the accessions could be grouped in to<br />
1 and 6 clusters. Figure 5, on the other hand, gave an estimate of 7 clusters using the<br />
cubic clustering criterion. In this case, there was no consensus that could be found<br />
among these criteria.<br />
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