THESIS

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Subcluster A is the only one accession of S. sanitwongsei collected in Thailand. This subcluster was separated from the other subcluster because it is the only one subcluster with upright plant growth habit and strong leaf blade lobbing, obtuse (~ 110°) leaf blade angle and very low yield per plant. Subcluster B is represented by TS02495 (S. indicum) collected from Thailand. The unique of this subcluster is for acute (~ 45°) leaf blade angle and very low yield per plant. Subcluster C is the homogenous of the two accessions of S. indicum collected from Laos, this can be separated from subcluster D by having the obtuse (~ 110°) leaf blade angle. Subcluster D is the largest subcluster with 5 accessions collected from Laos merging at 100 % similarity which. This cluster formed its own, separated stratified clusters. It could be recognized from the rest by very strong leaf blade lobbing, deep yellow fruit at ripening and very loose (spongy) fruit flesh density. Cluster VIII is a group of three accessions, belong to S. melongena collected from Laos. The unique of this cluster by having the purple fruit at ripening, dense fruit flesh density and grey yellow seed. Cluster IX is the only one accession, belong to S. mammosum collected from Malaysia. The unique of this cluster is strong leaf blade lobbing, acute (~ 45°) leaf blade angle, insert style exertion, slightly longer than broad of fruit length to breadth ratio (Appendix A), deep yellow fruit at harvesting, yellow orange fruit at maturity, average density of fruit flesh density and black seed. Hence, Cluster IX was considered to be the last cluster that fuses with the rest of the clusters. This indicated the cluster IX had nominal traits considering being almost different from the other clusters. 71
8 6 4 Figure 7 Cluster analysis of the 69 accessions, belonging to Solanum spp. based on the 11 nominal traits using UPGMA clustering method. SAC = S. aculeatissimum, SAE = S. aethiopicum, SF = S. ferox, SI = S. indicum, SM = S. melongena, SMA = S. mammosum, SS = S. sanitwongsei, ST = S. torvum, STR = S. trilobatum, SV = S. viarum, SX = S. xanthocarpum 2 TS03018 SI Laos TS02992 SI Laos TS02990 SI Laos TS03005 SI Laos TS02965 SI Laos VII TS02967 SI Laos TS02495 SI Thailand TS02491 SS Thailand TS02903 SF Malaysia TS02902 SF Malaysia TS00541 SF Thailand TS00426 SF Thailand TS00553 SF Thailand TS00563 SF Thailand TS01979 SF Thailand TS02062 SF Thailand TS02901 SF Malaysia VI TS02971 SF Laos TS02976 SF Laos TS03009 SF Laos TS03012 SF Laos TS03020 SF Laos TS02435 SF Thailand TS02978 SF Laos TS02989 SF Laos TS02728 ST Thailand TS02813 ST Laos TS02731 ST Thailand TS02877 ST Laos TS02862 ST Laos TS02937 ST Malaysia V TS02938 ST Malaysia TS02940 ST Malaysia TS02973 ST Laos TS02955 ST Cambodia TS01447A SV Thailand TS02445 STR Thailand IV TS01947 STR Thailand TS02739 STR Thailand III TS03029 SAE Laos TS02950 SX Cambodia TS00456 SM Thailand TS00455 SM Thailand TS00422A SX Thailand II TS02218 SM Thailand TS00422B SAC Thailand TS02816 SM Laos TS00551 SM Thailand TS02273B SM Vietnam TS01474B SM Thailand TS00555 SM Thailand TS00483 SX Thailand TS02440 SM Thailand TS01994 SM Thailand TS00552 SM Thailand TS00549 SM Thailand I TS02945 SX Malaysia TS00418 SX Thailand TS00417 SAC Thailand TS00487 SX Thailand TS00176 SM Thailand TS02441A SM Thailand TS00175 SM Thailand TS02217 SM Thailand TS00473 SM Thailand TS02815 SM Laos TS02812 SM Laos TS02822 SM Laos VIII TS02894 SMA Malaysia IX 0 72
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THESIS GENETIC DIVERSITY IN THE SOU
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ACKNOWLEDGEMENTS I would like to ex
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LIST OF TABLES Table Page 1 Ninety
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LIST OF FIGURES Figure Page 1 Selec
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LIST OF FIGURES (Continued) Figure
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LIST OF FIGURES (Continued) Appendi
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LIST OF ABBREVIATIONS cm = centimet
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Asian Vegetable Research and Develo
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OBJECTIVES 1. To characterize using
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campanulate, rotate or copular, mos
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Moreover, genetic diversity has an
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Therefore, germplasm characterizati
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some degree from each other and the
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Interspecific hybrids between wild
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Moreover, lines of S. melongena sho
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(qualitative) traits. The cluster a
Page 35 and 36: Plots of the clustering statistics
Page 37 and 38: Table 1 (Continued) Temporary No. S
Page 39 and 40: 2. Genetic relationships of Solanum
Page 41 and 42: pollen grains from randomly selecte
Page 43 and 44: RESULTS AND DISCUSSION In this stud
Page 45 and 46: Furthermore, TS02950 collected in C
Page 47 and 48: distinct from those species. Fruit
Page 49 and 50: yellow and globose. While, TS001447
Page 51 and 52: Table 4 Identification of 43 access
Page 53 and 54: Table 5 Re-identification of 5 acce
Page 55 and 56: accessions, hence, considered to ha
Page 57 and 58: Figure 1 Selected items from PROC U
Page 59 and 60: Table 7 Correlation coefficients sc
Page 61 and 62: N a m e o f V a r i a b l e o r C l
Page 63 and 64: 1.2.2 Cluster analysis I. Ratio sca
Page 65 and 66: P S E U D O F S T A T I S T I C Sto
Page 67 and 68: C U B I C C L U S T E R I N G C R I
Page 69 and 70: Table 9 Accessions number of Solanu
Page 71 and 72: Table 9 (Continued) Cluster Subclus
Page 73 and 74: Stratifying the accessions into 10
Page 75 and 76: (single fruit). It has also the wid
Page 77 and 78: were collected in Laos, while S. ma
Page 79 and 80: II. Nominal traits (qualitative tra
Page 81 and 82: Table 11 (Continued) Nominal traits
Page 83 and 84: The similarity coefficient values (
Page 85: (3 accessions). Subclaster A is cha
Page 89 and 90: Table 12 (Continued) Cluster Subclu
Page 91 and 92: However, the determination of the m
Page 93 and 94: This result is in agreement with th
Page 95 and 96: herbicide (atrazine) resistant trai
Page 97 and 98: 3) Cross - compatibility among S. a
Page 99 and 100: when the larger-flowered species we
Page 101 and 102: Table 14 Interspecific crossability
Page 103 and 104: Table 15 Interspecific crossability
Page 105 and 106: Table 17 Intra- and interspecific c
Page 107 and 108: Table 18 (Continued) Cross combinat
Page 109 and 110: A B C D E F G H Figure 8 The hybrid
Page 111 and 112: Figure 10 F1 hybrid plant from S. m
Page 113 and 114: Confirmation of interspecific hybri
Page 115 and 116: However, pollen fertility of the in
Page 117 and 118: Table 19 The pollen fertility of F1
Page 119 and 120: Table 21 (Continued) Species Access
Page 121 and 122: S00860) showed a same ploidy level
Page 123 and 124: Moreover, an accurate determination
Page 125 and 126: S. americanum (S00269) (2n) S. amer
Page 127 and 128: S. villosum (S00854) (4n) S. villos
Page 129 and 130: S. villosum (S00860) (4n) S. villos
Page 131 and 132: Moreover, the crosses between S. am
Page 133 and 134: S. melongena (S00625) S. melongena
Page 135 and 136: S. melongena (S00809) F1 S. villosu
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S. americanum (S00861) S. americanu
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S. americanum (S00269) S. americanu
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S. villosum (TS02600) F1 S. villosu
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S. villosum (S00854) F1 S. american
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S. nigrum (TS02930) F1 S. americanu
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132 For the interspecific crossing
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S. nigrum). For the interspecific c
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, and M. Ahmand.1989. Morphological
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138 Collonnier, C., I. Fock, V. Kas
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and J.A. Chweya. 1997. Black Nights
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and . 1988b. Organelle composition
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Laban, J.C.G. 2003. Genetic Diversi
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spinous Solanums. Theoret. Appl. Ge
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Singh, J. and M. Rai. 2005. Eggplan
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APPENDICES 150
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SEEDLING DATA AVRDC-GRSU CHARACTERI
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S300 Leaf blade tip angle 1 = Very
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S530 Fruit length/breadth ratio 1 =
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S640 Fruit calyx length (N=10) ____
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Appendix Table A1 Characterization
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Appendix Table A1 (Continued) Tempo
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Appendix Table A4 List of morpholog
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Appendix Figure A2 Morphological ch
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Appendix Figure A8 Morphological ch
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Appendix B Identification of specie
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Moments N 91 Sum Weights 91 Mean 7.
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Moment N 91 Sum Weights 91 Mean 101
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Moments N 91 Sum Weights 91 Mean 10
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THESIS

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