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Table 1 (Continued) Temporary No. Species Country of origin TS02938 S. torvum Malaysia TS02939 S. torvum Malaysia TS02940 S. torvum Malaysia TS02941 S. torvum Malaysia TS02945 S. xanthocarpum Malaysia TS02946 S. xanthocarpum Malaysia TS02950 S. macrocarpon Cambodia TS02955 S. torvum Cambodia TS02965 S. sisymbrifolium Laos TS02967 S. ferox Laos TS02971 S. ferox Laos TS02973 S. torvum Laos TS02976 S. sisymbrifolium Laos TS02978 S. ferox Laos TS02989 S. ferox Laos TS02990 S. sisymbrifolium Laos TS02992 S. sisymbrifolium Laos TS03005 S. sisymbrifolium Laos TS03009 S. ferox Laos TS03012 S. ferox Laos TS03018 S. sisymbrifolium Laos TS03020 S. ferox Laos TS03029 S. aethiopicum Laos TS03049 S. melongena Cambodia TS03050 S. melongena Cambodia TS03051 S. melongena Cambodia TS03052 S. melongena Cambodia TS03053 S. melongena Cambodia 23
2. Genetic relationships of Solanum spp. using interspecific hybridization Five collections of Solanum species at GRSU, AVRDC (Table 2) comprised of four accessions of S. melongena (S00022, S00388, S00625 and S00809), one accession each of S. torvum (S00429) and S. nigrum (TS02930), four accessions of S. americanum (S00269, S00859, S00861 and S00865) and three accessions of S. villosum were sown in seedling trays in the greenhouse. Seedlings were transplanted into black plastic pots (42 cm in diameter and 30 cm heigh) after 30 days after sowing (5 pots for each accession). Young flower buds of female parents were emasculated and bagged in a glassine bag, two days before anthesis at flowering stage to prevent self-pollination. Pollens were collected and stored at 4-5 °C. Hand-pollination was done when the stigma was receptive, then re-bagged and labeled. The pollination was done for 2 days after being emasculated and the crosses were made during 8.00-11.00 A.M. The intraand interspecific crosses pollinations were made in 169 crossing combinations: 1. S. melongena x S. melongena 2. S. melongena x S. torvum 3. S. melongena x S. americanum 4. S. melongena x S. villosum 5. S. melongena x S. nigrum 6. S. torvum x S. torvum 7. S. torvum x S. americanum 8. S. torvum x S. villosum 9. S. torvum x S. nigrum 10. S. americanum x S. americanum 11. S. americanum x S. villosum 12. S. americanum x S. nigrum 13. S. villosum x S. villosum 14. S. nigrum x S. nigrum and their reciprocal crossing. 24
Page 1: THESIS GENETIC DIVERSITY IN THE SOU
Page 5 and 6: ACKNOWLEDGEMENTS I would like to ex
Page 7 and 8: LIST OF TABLES Table Page 1 Ninety
Page 9 and 10: LIST OF FIGURES Figure Page 1 Selec
Page 11 and 12: LIST OF FIGURES (Continued) Figure
Page 13 and 14: LIST OF FIGURES (Continued) Appendi
Page 15 and 16: LIST OF ABBREVIATIONS cm = centimet
Page 17 and 18: Asian Vegetable Research and Develo
Page 19 and 20: OBJECTIVES 1. To characterize using
Page 21 and 22: campanulate, rotate or copular, mos
Page 23 and 24: Moreover, genetic diversity has an
Page 25 and 26: Therefore, germplasm characterizati
Page 27 and 28: some degree from each other and the
Page 29 and 30: Interspecific hybrids between wild
Page 31 and 32: Moreover, lines of S. melongena sho
Page 33 and 34: (qualitative) traits. The cluster a
Page 35 and 36: Plots of the clustering statistics
Page 37: Table 1 (Continued) Temporary No. S
Page 41 and 42: pollen grains from randomly selecte
Page 43 and 44: RESULTS AND DISCUSSION In this stud
Page 45 and 46: Furthermore, TS02950 collected in C
Page 47 and 48: distinct from those species. Fruit
Page 49 and 50: yellow and globose. While, TS001447
Page 51 and 52: Table 4 Identification of 43 access
Page 53 and 54: Table 5 Re-identification of 5 acce
Page 55 and 56: accessions, hence, considered to ha
Page 57 and 58: Figure 1 Selected items from PROC U
Page 59 and 60: Table 7 Correlation coefficients sc
Page 61 and 62: N a m e o f V a r i a b l e o r C l
Page 63 and 64: 1.2.2 Cluster analysis I. Ratio sca
Page 65 and 66: P S E U D O F S T A T I S T I C Sto
Page 67 and 68: C U B I C C L U S T E R I N G C R I
Page 69 and 70: Table 9 Accessions number of Solanu
Page 71 and 72: Table 9 (Continued) Cluster Subclus
Page 73 and 74: Stratifying the accessions into 10
Page 75 and 76: (single fruit). It has also the wid
Page 77 and 78: were collected in Laos, while S. ma
Page 79 and 80: II. Nominal traits (qualitative tra
Page 81 and 82: Table 11 (Continued) Nominal traits
Page 83 and 84: The similarity coefficient values (
Page 85 and 86: (3 accessions). Subclaster A is cha
Page 87 and 88: 8 6 4 Figure 7 Cluster analysis of
Page 89 and 90:
Table 12 (Continued) Cluster Subclu
Page 91 and 92:
However, the determination of the m
Page 93 and 94:
This result is in agreement with th
Page 95 and 96:
herbicide (atrazine) resistant trai
Page 97 and 98:
3) Cross - compatibility among S. a
Page 99 and 100:
when the larger-flowered species we
Page 101 and 102:
Table 14 Interspecific crossability
Page 103 and 104:
Table 15 Interspecific crossability
Page 105 and 106:
Table 17 Intra- and interspecific c
Page 107 and 108:
Table 18 (Continued) Cross combinat
Page 109 and 110:
A B C D E F G H Figure 8 The hybrid
Page 111 and 112:
Figure 10 F1 hybrid plant from S. m
Page 113 and 114:
Confirmation of interspecific hybri
Page 115 and 116:
However, pollen fertility of the in
Page 117 and 118:
Table 19 The pollen fertility of F1
Page 119 and 120:
Table 21 (Continued) Species Access
Page 121 and 122:
S00860) showed a same ploidy level
Page 123 and 124:
Moreover, an accurate determination
Page 125 and 126:
S. americanum (S00269) (2n) S. amer
Page 127 and 128:
S. villosum (S00854) (4n) S. villos
Page 129 and 130:
S. villosum (S00860) (4n) S. villos
Page 131 and 132:
Moreover, the crosses between S. am
Page 133 and 134:
S. melongena (S00625) S. melongena
Page 135 and 136:
S. melongena (S00809) F1 S. villosu
Page 137 and 138:
S. americanum (S00861) S. americanu
Page 139 and 140:
S. americanum (S00269) S. americanu
Page 141 and 142:
S. villosum (TS02600) F1 S. villosu
Page 143 and 144:
S. villosum (S00854) F1 S. american
Page 145 and 146:
S. nigrum (TS02930) F1 S. americanu
Page 147 and 148:
132 For the interspecific crossing
Page 149 and 150:
S. nigrum). For the interspecific c
Page 151 and 152:
, and M. Ahmand.1989. Morphological
Page 153 and 154:
138 Collonnier, C., I. Fock, V. Kas
Page 155 and 156:
and J.A. Chweya. 1997. Black Nights
Page 157 and 158:
and . 1988b. Organelle composition
Page 159 and 160:
Laban, J.C.G. 2003. Genetic Diversi
Page 161 and 162:
spinous Solanums. Theoret. Appl. Ge
Page 163 and 164:
Singh, J. and M. Rai. 2005. Eggplan
Page 165 and 166:
APPENDICES 150
Page 167 and 168:
SEEDLING DATA AVRDC-GRSU CHARACTERI
Page 169 and 170:
S300 Leaf blade tip angle 1 = Very
Page 171 and 172:
S530 Fruit length/breadth ratio 1 =
Page 173 and 174:
S640 Fruit calyx length (N=10) ____
Page 175 and 176:
Appendix Table A1 Characterization
Page 177 and 178:
Appendix Table A1 (Continued) Tempo
Page 179 and 180:
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Appendix Table A4 List of morpholog
Page 203 and 204:
Appendix Figure A2 Morphological ch
Page 205 and 206:
Appendix Figure A8 Morphological ch
Page 207 and 208:
Appendix B Identification of specie
Page 209 and 210:
Moments N 91 Sum Weights 91 Mean 7.
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Moment N 91 Sum Weights 91 Mean 101
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Moments N 91 Sum Weights 91 Mean 10
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