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Interspecific hybrids between wild and cultivated of S. melongena species<br />

have been successful in only a few cases. Such as S. melongena x S. aethipoicum<br />

(Daunay et al., 1993), S. melongena x S. indicum (Rao and Kumar, 1980; Rao and<br />

Rao, 1984; Patel, 2001), S. melongena x S. sodomeum (Tudor and Tomescu, 1995), S.<br />

melongena x S. macrocarpon (Schaff et al., 1982), S. melongena x S. insanum (Rao<br />

and Rao, 1984), S. melongena x S. gilo (Kashyap et al., 2003) and S. melongena x S.<br />

integrifolium (Rao and Baksh, 1979).<br />

Moreover, embryo rescue was successfully used to recover hybrids of S.<br />

melongena with S. khasianum (Sharma et al., 1980), S. sisymbrifolium (Sharma et al.,<br />

1984; Blestsos et al., 1998) and S. torvum (Daunay et al., 1991; Blestsos et al., 1998)<br />

but these hybrids were sterile. Fertility was reported in hybrids of S. melongena with<br />

S. macrocarpon (Gowda et al., 1990) and S. torvum (Daunay et al., 1991) when<br />

diploid hybrids (2x) were brought to the amphiploid status (4x) by colchicines<br />

treatment. However, the successful in interspecific crosses have been obtained with<br />

only few wild species. In such attempted, the hybrids have been developed through<br />

embryo rescue. In addition, such hybrids have either been sterile or have had very low<br />

pollen fertility. This may be due to pre- and post- pollination effects (Kashyap et al.,<br />

2003).<br />

Nevertheless, interspecific crosses between S. melongena and other Solanum<br />

species, bearing interesting agronomical traits, have sometimes been limited by sexual<br />

barriers (Collonnier et al., 2001). Crossability between S. melongena and species of<br />

other genera or distant subgenera such as subgenus Archaesolanum, subgenus Potatoe<br />

or subgenus Solanum is very low (Daunay et al., 1991). This may result from lack of<br />

genetic information in one partner about the other, due to evolutionary divergence;<br />

this is known as incongruity (Franklin et al., 1995).<br />

However, plant regeneration from protoplast has been achieved in both<br />

cultivated and wild species of S. melongena. The protoplast culture and somatic<br />

hybridization would be useful in overcoming the pre- and post- fertilization breeding<br />

barriers encountered during conventional breeding. Further, protoplast cultures are<br />

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