THESIS

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Resources (IBPGR), Rome, Italy. Islam, O. 1992. Genetic resources and their role in horticultural crop improvement, pp. 18-22. In M.L. Chadha, A.M.K. Amzd Hossain and S.M. Mono war Hossain, eds. Germplasm Collection, Evaluation, Documentation and Conservation. BARI, Joydebpur, Gazipur, Bangladesh. Isshiki, S. and N. Kawajiri. 2002. Effect of cytoplasm of Solanum violaceum Ort. on fertility of eggplant (S. melongena L.). Scientia Horticulturae 93: 9-18. Iwamoto, Y., M. Hirai, N. Ohmido, K. Fukui and H. Ezura. 2007. Fertile somatic hybrids between Solanum integrifolium and S. sanitwongsei (syn. S. kurzii) as candidates for bacterial wilt-resistant rootstock of eggplant. Plant Biotechnology 24: 179-184. Jansky, S. 2006. Review overcoming hybridization barriers in potato. Plant Breeding 125: 1-12. 143 Jarl, E., M. Rietveld and J.M. de Haas. 1999. Transfer of fungal tolerance through interspecific somatic hybridisation between Solanum melongena and S. torvum. Plant Cell Reports 18: 791-796. Kalda, T.S., V. Swarup and B. Choudhury. 1977. Resistance to phomopsis blight in eggplant. Veg. Sci. 4: 90-101. Kashyap, V., S.V. Kumar, C. Collonnier, F. Fusari, R. Haicour, G.L. Rotina, D. Sihachakr and M.V. Rajam. 2003. Review: biotechnology of eggplant. Scientia Horticulturae 97: 1-25. Khan, R., G.R. Rao and N.H. Siddiqui. 1978. Role of the tetraploid Solanum nigrum L. and S. americanum Mill. in the origin and evolution of indian hexaploid S. nigrum. Acta Botanica India 6 (Suppl): 161-164.
Laban, J.C.G. 2003. Genetic Diversity of Seven Solanum Species in the Asian Vegetable Research and Development Center (AVRDC) Gene Bank Based on Random Amplified Polymorphic DNA (RAPD) Markers. B.S. Thesis, University of the Philippines Los Baños. Philippines. Lee, H.C. and T.Y. Lin. 2005. Isolation of plant nuclei suitable for flow cytometry from recalcitrant tissue by use of a filtration column. Plant Molecular Biology Reporter 23: 53-58. Lester, R.N. 1986. Taxonomy of scarlet eggplants, S. aethiopicum L. Acta Horticulturae 182: 125-132. 144 and J.H. Kang. 1998. Embryo and endosperm function and failure in Solanum species and hybrids. Annals of Botany 82: 445-453. Loveless, M.D. and J.L. Hamrick. 1984. Ecological determinants of genetic structure in plant populations. Annu. Rev. Ecol. Syst. 15: 65-95. Manigbas, K.F. 2005. Morphological Diversity of Solanum nigrum Complex at the Asian Vegetable Research and Development (AVRDC) Taiwan. B.S. Thesis, University of the Philippines Los Baños. Philippines. Marfil, C.F., R.W. Masuelli, J. Davison and L. Comai. 2006. Genomic instability in Solanum tuberosum x Solanum kurtzianm interspecific hybrids. Genome 49: 104-113. Martinez, C.P., K. Arumuganathan, H. Kikuchi and E.D. Earle. 1994. Nuclear DNA content of ten rice species as determined by flow cytometry. Jpn. J. Genet. 69: 513-523. McCammon, K.R. and S. Honma. 1983. Morphological and cytogenetic analysis of an interspecific hybrids eggplant S. melongena x S. torvum. HortScience 18
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THESIS GENETIC DIVERSITY IN THE SOU
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ACKNOWLEDGEMENTS I would like to ex
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LIST OF TABLES Table Page 1 Ninety
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LIST OF FIGURES Figure Page 1 Selec
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LIST OF FIGURES (Continued) Figure
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LIST OF FIGURES (Continued) Appendi
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LIST OF ABBREVIATIONS cm = centimet
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Asian Vegetable Research and Develo
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OBJECTIVES 1. To characterize using
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campanulate, rotate or copular, mos
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Moreover, genetic diversity has an
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Therefore, germplasm characterizati
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some degree from each other and the
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Interspecific hybrids between wild
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Moreover, lines of S. melongena sho
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(qualitative) traits. The cluster a
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Plots of the clustering statistics
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Table 1 (Continued) Temporary No. S
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2. Genetic relationships of Solanum
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pollen grains from randomly selecte
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RESULTS AND DISCUSSION In this stud
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Furthermore, TS02950 collected in C
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distinct from those species. Fruit
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yellow and globose. While, TS001447
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Table 4 Identification of 43 access
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Table 5 Re-identification of 5 acce
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accessions, hence, considered to ha
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Figure 1 Selected items from PROC U
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Table 7 Correlation coefficients sc
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N a m e o f V a r i a b l e o r C l
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1.2.2 Cluster analysis I. Ratio sca
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P S E U D O F S T A T I S T I C Sto
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C U B I C C L U S T E R I N G C R I
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Table 9 Accessions number of Solanu
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Table 9 (Continued) Cluster Subclus
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Stratifying the accessions into 10
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(single fruit). It has also the wid
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were collected in Laos, while S. ma
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II. Nominal traits (qualitative tra
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Table 11 (Continued) Nominal traits
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The similarity coefficient values (
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(3 accessions). Subclaster A is cha
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8 6 4 Figure 7 Cluster analysis of
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Table 12 (Continued) Cluster Subclu
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However, the determination of the m
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This result is in agreement with th
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herbicide (atrazine) resistant trai
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3) Cross - compatibility among S. a
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when the larger-flowered species we
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Table 14 Interspecific crossability
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Table 15 Interspecific crossability
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Table 17 Intra- and interspecific c
Page 107 and 108: Table 18 (Continued) Cross combinat
Page 109 and 110: A B C D E F G H Figure 8 The hybrid
Page 111 and 112: Figure 10 F1 hybrid plant from S. m
Page 113 and 114: Confirmation of interspecific hybri
Page 115 and 116: However, pollen fertility of the in
Page 117 and 118: Table 19 The pollen fertility of F1
Page 119 and 120: Table 21 (Continued) Species Access
Page 121 and 122: S00860) showed a same ploidy level
Page 123 and 124: Moreover, an accurate determination
Page 125 and 126: S. americanum (S00269) (2n) S. amer
Page 127 and 128: S. villosum (S00854) (4n) S. villos
Page 129 and 130: S. villosum (S00860) (4n) S. villos
Page 131 and 132: Moreover, the crosses between S. am
Page 133 and 134: S. melongena (S00625) S. melongena
Page 135 and 136: S. melongena (S00809) F1 S. villosu
Page 137 and 138: S. americanum (S00861) S. americanu
Page 139 and 140: S. americanum (S00269) S. americanu
Page 141 and 142: S. villosum (TS02600) F1 S. villosu
Page 143 and 144: S. villosum (S00854) F1 S. american
Page 145 and 146: S. nigrum (TS02930) F1 S. americanu
Page 147 and 148: 132 For the interspecific crossing
Page 149 and 150: S. nigrum). For the interspecific c
Page 151 and 152: , and M. Ahmand.1989. Morphological
Page 153 and 154: 138 Collonnier, C., I. Fock, V. Kas
Page 155 and 156: and J.A. Chweya. 1997. Black Nights
Page 157: and . 1988b. Organelle composition
Page 161 and 162: spinous Solanums. Theoret. Appl. Ge
Page 163 and 164: Singh, J. and M. Rai. 2005. Eggplan
Page 165 and 166: APPENDICES 150
Page 167 and 168: SEEDLING DATA AVRDC-GRSU CHARACTERI
Page 169 and 170: S300 Leaf blade tip angle 1 = Very
Page 171 and 172: S530 Fruit length/breadth ratio 1 =
Page 173 and 174: S640 Fruit calyx length (N=10) ____
Page 175 and 176: Appendix Table A1 Characterization
Page 177 and 178: Appendix Table A1 (Continued) Tempo
Page 201 and 202: Appendix Table A4 List of morpholog
Page 203 and 204: Appendix Figure A2 Morphological ch
Page 205 and 206: Appendix Figure A8 Morphological ch
Page 207 and 208: Appendix B Identification of specie
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Moments N 91 Sum Weights 91 Mean 7.
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Moment N 91 Sum Weights 91 Mean 101
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Moments N 91 Sum Weights 91 Mean 10
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