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Chapter
4
Figure 4-5: PNA microarray analysis with Cy5-oligonucletides in different combinations ( see Table
4-2) to simulate all the possible genotypes.
Six clearly different hybridization patterns were obtained, thus demonstrating that arginine-
based PNAs are very efficient
in discriminating single point mismatches also when deposited
on the solid surface. These data demonstrate that the arginine-PNA three ApoE isoforms, thus providing a
microarrays could easily
discriminate among
different genotypes related to the
potential way to rapidly assesss the genetic
predisposition to the Alzheimer disease.
4.3 Conclusions
In this work we have demonstrated thatt the arginine-based PNAs synthesized carrying one
chiral monomer with two stereogenic centers of suitable configuration in
the middle of the
strand, are very efficient in discriminating single point mutations both in
solutions and on a
solid surface. Microarrays built by using four arginine-PNAs were shown to be able to
discriminate the presence or the absence of their complementary oligonucleotides
also in
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