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Chapter 4 4.4.6 Microarrays analysis Pictures of the PNA microarrays were recorded by using a scanner ScanArray Express 20 (Perkin Elmer, Waltham, USA). Laser was set at excitation wavelenght of 646nm and emission at 664nm, for Cy5. Pictures were acquired with laser power at 100% and photomultiplier at 40%. 4.5 References 1 A.J. Brookes, Gene, 1999, 234, 177 2 G. Ultermann; U. Langebeck; U. Beisiegel; W. Weber, Am., J. Hum. Genet., 1980, 32; 921 3 K. Kontula, K. Aalto-Setălă, T. Kuusi, L. Hămălăinen, A. Syvănen, Clin. Chem., 1990, 36. 12, 2087 4 Corder E. H., Saunders A. M., Risch N. J. et al., Nature Genetics, 1994, 7, 180 5 A. Borenstein, R., C.I. Copenhaver, J.A. Mortimer, Alzheimer Dis.Assoc.Disord., 2006, 20, 2006, 63 6 A.M. Saunders, C. Hulette, K.A. WelshBohmer, D.E. Schmechel, B. Crain B, J.R. Burke, M.J. Alberts, W.J. Strittmatter, J.C.S. Breitner, C. Rosenberg, S.V. Scott, P.C. Gaskell, M.A. PericakVance, A.D. Roses, The Lancet, 1996, 348, 90 7 K. Sobin, M. Ashish, Annu. Rev. Biomed., 2007, 9, 289 8 J. Kian-Kon, Wen-Tso Liu, Anal. Bioanal. Chem., 2006, 386, 427 9 A. Sassolas, B. D. Leca-Bouvier, L. J. Blum, Chem. Rev., 2008, 108, 109 10 M. B. Miller, Yi-Wei Tang, Clinical Microbiology Reviews, 2009, 22, 4, 611 11 E.M. Southern, Electrophoresis, 1995, 16, 1539 12 J. Weiler, H. Gausepohl, N. Hauser, O. N. Jensen, J. D. Hoheisel, Nucleic Acids Res., 1997, 25, 2792 13 P.E. Nielsen, M., Egholm, R.H. Berg, O. Buchardt, Science, 1991, 254, 1497 14 O. Brandt, J. D. Hoheisel, Trends Biotechnol., 2004, 22, 617 15 A. Germini, A. Mezzelani, F. Lesignoli, R. Corradini, R. Marchelli, R. Bordoni, C. Consolandi, G. De Bellis, J. Agric. Food Chem., 2004, 52, 4535 16 Z. C. Liu, D.S. Shin, M. Shokouhimehr, K. N. Lee, B. W. Yoo, Y. K. Kim, Y. S. Lee, Biosens. Bioelectron., 2007, 22, 12, 2891 17 F. R. Raymond, H. Hoang-Ann, R. Peytavi, L. Bissonnette, M. Boissinot, F. J. Picard, M. Leclerc and M. Bergeon, BMC Biotechnology, 2005, 5, 10 18 J. K. Pokorski, J. M. Nam, R. A. Vega, C. A. Mirkin, D. H. Appella, Chem. Comm, 2005, 16, 2101 19 J. Choi, C. Kim, H. Park, Journal of Clinical Microbiology, 2009, 47, 6, 1785 20 O. Brandt, J. Feldner, A. Stephan, Nucleic Acids Research, 2003, 31, 19,1 21 O. Brandt, J. Feldner, S. Hellweg et al., Applied Surface Science, 2006, 252, 6935 22 C. Sun, B. S Gaylord, J. W Hong, B. Liu, G. C Bazan, Nature Protocols, 2007, 2, 9, 2149 23 F. He, F. Feng, S. Wang, Trends in Biotechnology, 2007, 26, 2, 57 24 J. K. Pokorski, J. Nam, R. A. Vega, C. A. Mirkin, D. H. Appella, Chem. Commun., 2005, 2101 25 R. Corradini, S. Sforza, T. Tedeschi, F. Totsingan, R. Marchelli, Curr. Top. Med. Chem., 2007, 7, 681 26 S. Sforza, T. Tedeschi, R. Corradini, R. Marchelli, Eur. J. Org. Chem., 2007, 35, 5879. 27 A. Germini, S. Rossi, A. Zanetti, R. Corradini, C. Fogher, R. Marchelli, J. Agric. Food Chem., 2005, 53, 3958 28 S. Sforza, T. Tedeschi, R. Corradini, D. Ciavardelli, A. Dossena, R. Marchelli, Eur. J. Org. Chem., 2003, 6, 1056 29 C. Sun, B.S. Gaylord, J. W. Hong, B.Liu, G.C. Bazan, Nature Protocols, 2007, 2, 2148 88
Arg-PNA Microarrays 30 T. Tedeschi, S. Sforza, R. Corradini, R. Marchelli, Tetrahedron Lett., 2005, 46, 8395 31 S.A. Thomson, J.A. Josey, R. Cadilla, M.D. Gaul, C.F. Hassman, M.J. Luzzio, A.J. Pipe, K.I. Reed, D.J. Ricca, R.W. Wiethe, S.A. Noble, Tetrahedron, 1995, 51, 6179 89
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Università degli studi di Parma Do
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2.4.4 Fluorescence measurements ...
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Chapter 6 The double nature of Pept
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Preface In Chapter 2 a particular t
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Chapter 1 PNAs: From birth to adult
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PNAs: from birth to adulthood one,
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PNAs: from birth to adulthood betwe
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PNAs: from birth to adulthood explo
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PNAs: from birth to adulthood well,
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PNAs: from birth to adulthood + Res
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PNAs: from birth to adulthood repet
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PNAs: from birth to adulthood 1.6 M
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PNAs: from birth to adulthood amino
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PNAs: from birth to adulthood Cycli
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PNAs: from birth to adulthood DNA a
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PNAs: from birth to adulthood 1.7 C
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PNAs: from birth to adulthood Submo
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PNAs: from birth to adulthood beaco
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PNAs: from birth to adulthood DNA s
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PNAs: from birth to adulthood this
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Page 45 and 46: Chapter 2 Label-free detection of S
Page 47 and 48: PNA Molecular Beacons Figure 2-1: S
Page 49 and 50: PNA Molecular Beacons DNA is added,
Page 51 and 52: PNA Molecular Beacons the affinitie
Page 53 and 54: PNA Molecular Beacons In particular
Page 55 and 56: PNA Molecular Beacons The sample th
Page 57 and 58: PNA Molecular Beacons pipetting to
Page 59 and 60: PNA Molecular Beacons The mastermix
Page 61 and 62: Chapter 3 A new class of Chiral PNA
Page 63 and 64: Arginine-PNAs monomers in the middl
Page 65 and 66: Arginine-PNAs The synthesis of the
Page 67 and 68: Arginine-PNAs yield in this reactio
Page 69 and 70: Arginine-PNAs (increased electrosta
Page 71 and 72: Arginine-PNAs chain + Boc methyl gr
Page 73 and 74: Arginine-PNAs foam. Yield: 30%. 1 H
Page 75 and 76: Arginine-PNAs was allowed to stir f
Page 77 and 78: Arginine-PNAs PNAs was carried out
Page 79 and 80: Chapter 4 Chiral PNA application on
Page 81 and 82: Arg-PNA Microarrays a surface. Alth
Page 83 and 84: Arg-PNA Microarrays 4.2 Results and
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Page 87 and 88: Arg-PNA Microarrays PNA-microarray
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Page 91 and 92: Arg-PNA Microarrays complex mixture
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Page 98 and 99: Chapter 5 5.1 Introduction The incr
Page 100 and 101: Chapter 5 be printed, in order to m
Page 102 and 103: Chapter 5 Table 5-1. PNA sequences
Page 104 and 105: Chapter 5 printing the PNA on a gla
Page 106 and 107: Chapter 5 22.4°C 24.0°C 25.5°C 2
Page 108 and 109: Chapter 5 PNA 3 PNA 5 PNA 6 Slide 1
Page 110 and 111: Chapter 5 times. The substrates wer
Page 112 and 113: Chapter 5 chemicals were used as re
Page 114 and 115: Chapter 5 5.4.6 Microcontact Printi
Page 116 and 117: Chapter 5 5.5 References 1 A. L. Be
Page 118 and 119: Chapter 6 6.1 Introduction The use
Page 120 and 121: Chapter 6 Among all the peptides us
Page 122 and 123: Chapter 6 Figure 6-2: A standard PN
Page 124 and 125: Chapter 6 peptide 1 and the PNA tri
Page 126 and 127: Chapter 6 6.2.2 Uptake experiments
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Page 130 and 131: Chapter 6 1.41 (s, 9H, Boc methyl g
Page 132 and 133: Chapter 6 Arg + CHα Lys), 4.0-4.6
Page 134 and 135: Chapter 6 12 B. P. Gangamani, V. A.
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