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Chapter 5
Peptide Nucleic Acid microcontact printing for the
fabrication of microarrays
In this chapter an advanced technique for surface derivatization is reported. Microcontact
Printing is a widely used technique to pattern surfaces with micrometer features, allowing to
obtain well-defined and homogeneous patterns in a shorter time than standard methods.
Surface patterning with PNAs and characterization of the surface is shown in the first part.
Hybridization studies with complementary and mismatch TAMRA labeled oligonucleotides is
shown in the second part. Finally the system is combined with a commercial arrayer in order
to create a PNA-microarray by microcontact printing, for the fabrication of arrays that are
able to recognize DNA on surface with high selectivity. The work reported in this section has
been carried out at the University of Twente, The Netherlands, in the group of Molecular
Nanofabrication (MNF).
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