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Fig. 3.7. Bippinaria larvae of Marthasterias glacialis observed unaltered after 24 h of
being cultured at 200 atm and 10° C. (Micrograph by Francisco Benitez)
3.3- Results
3.3.1- Temperature/pressure effects on fertilized eggs.
Asterias rubens
At 6 h: At 5°C there was almost no evidence of cleavage at any pressure (Fig.
3.8). At 10°C and 1atm, ~40 % of embryos reached the eight- and ~50 % reached the
16-cell stage, at 50 and 100atm around 20% of the embryos were at 4-, 8-, and 16-
cell, and ~70 % remained uncleaved, at 150 and 200atm between 80 and 90 % of the
zygotes were uncleaved and between 10 and 20 % had an abnormal development. At
15°C and 1 atm around 40 % of the embryos were 16 cell- and ~ 40 % were at 32-cell,
less than 20 % of them were abnormal, at 50atm about 50 % of them were 16-cell,
almost 20 % were 32-cell and nearly 40 % were abnormal embryos, at 100 and
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