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Download (3398Kb) - ePrints Soton - University of Southampton

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Data on maximum body size, maximum egg size and maximum Gonad Index,<br />

were plotted against depth <strong>of</strong> maximum abundance for all the species, and the<br />

resulting graphs were investigated in order to find possible patterns or relations<br />

between the reproductive features. The degree to which the variables were related was<br />

tested by using the Spearman Rank<br />

Correlation Coefficient (Rs) and the results discussed (Fowler et al., 2000). A nonparametric<br />

test was preferred because the data were presented as actual observations,<br />

observations converted to ranks and indices; furthermore a parametric test would<br />

require the data to be normally distributed and to have homogeneous variances<br />

(Fowler et al., 2000).<br />

Although the relatively low number <strong>of</strong> data would suggest that the data set is<br />

not suitable for a multivariate analysis, this analysis was performed with the PRIMER<br />

(Plymouth Routines in Multivariate Ecological Research) Version 5 programme<br />

(Clarke and Warwick, 2001) to investigate if a pattern could be observed.<br />

Data on adult size, egg size and Gonad Index were analyzed together by the<br />

Normalized Euclidean distance measure applied to all the asteroid species.<br />

Hierarchical clustering with group-averaged linking and non-metric multidimensional<br />

scaling using all resulting similarity matrices was performed (Clarke and<br />

Warwick, 2001). The groupings identified by both cluster analysis and the MDS plots<br />

were investigated using the available data on the species regarding reproductive<br />

features determined in this study and depth related distribution in the NE Atlantic<br />

established by Howell et al. 2002.<br />

4.3- Results<br />

The Spearman’s correlation coefficient showed that no correlation exists<br />

between depth (over the entire range) and body size (as R) <strong>of</strong> the species (Rs= 0.056,<br />

75

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