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blastulae, at 200atm 90% of the embryos were abnormal; the rest were uncleaved
(Fig. 3.13).
percentage
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5 o C
10 o C
15 o C
U 2 4 8 32 BA U 2 4 8 32 BA U 2 4 8 32 BA
20 o C
U 2 4 8 32 BA
200 atm 150 atm 100 atm 50 atm 1 atm
D ev elo p m en tal S tag e
Fig. 3.13. Marthasterias glacialis embryos incubated at 5, 10, 15 and 20° C at 1, 50,
100, 150 and 200 atm for 12 h. Histogram represent % mean and SD. Development
stages are (U) Uncleaved, 2 to 32 cell, (B) Blastula and (A) Abnormal.
At 24 h: At 5°C and 1atm ~80 % of embryos were abnormal, ~20 % were
uncleaved, at 50 and 100atm between 70 and 80 % were uncleaved, between 20 and
30 % were abnormal, at 150atm ~90 % of embryos were abnormal, the rest were
uncleaved, at 200atm all the embryos were abnormal. At 10°C and 1atm ~40 % were
blastula, ~40 % were 16- and 32-cell, ~20% were abnormal, at 50atm ~80 % were
blastulae, ~20 % were abnormal, at 100atm ~40 % were 64-cell, ~10 % were
blastulae, ~25% were abnormal and ~25 % were 32- cell, at 150atm ~70% of the
embryos were abnormal, around 30 % was a mixture of 8-, 16-, 32- and 64-cell, at
200atm ~70 % were abnormal, the rest were uncleaved. At 15°C and 1 and 50atm ~80
% of embryos were blastulae, the rest were uncleaved; at 100atm ~50 % were
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