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Marthasterias glacialis (ECHINODERMATA: ASTEROIDEA) POTENTIAL FOR<br />

DEEP-SEA INVASION FROM THE NORTH ATLANTIC.<br />

3.1. Introduction ………………………………………………………………..…….35<br />

3.1.1. Chapter objectives ……………………………………………………..………41<br />

3.2. Material and methods ……………………………………………………………..41<br />

3.2.1. Field sampling and spawning …………………………………………………….41<br />

3.2.2. Temperature/pressure effects on fertilized eggs …………………………………...42<br />

3.2.3. Temperature/pressure effects on larvae ……………………………………………45<br />

3.3. Results ………………………………………………………………………………46<br />

3.3.1. Temperature/pressure effects on fertilized eggs …………………………….…..…46<br />

3.3.2. Temperature/pressure effects on swimming bippinaria at 24 h …………….……54<br />

3.4. Discussion ………………………………………………………………………………55<br />

3.5. Recommendations………………………………………………………………………60<br />

CHAPTER FOUR – REPRODUCTIVE FEATURES OF ASTEROIDS IN THE<br />

PORCUPINE SEABIGHT AND PORCUPINE ABYSSAL PLAIN, N.E. ATLANTIC<br />

AND THEIR RELATION TO DEPTH DISTRIBUTION OF THE SPECIES<br />

4.1. Introduction ……………………………………………………………………...62<br />

4.1.1 Fecundity and egg size ……………………………………………………………..63<br />

4.1.2. Body size ………………………………………………………………………………68<br />

4.2. Material and methods …………………………………………………………..…69<br />

4.2.1. Collection <strong>of</strong> data ……………………………………………………………………...69<br />

4.2.2. Histology ………………………………………………………………………………72<br />

4.2.3. Image analysis and estimation <strong>of</strong> fecundity …………………………….……..72<br />

4.2.4. Statistical analysis ………………………………………………………..……75<br />

4.3. Results ………………………………………………………………………………76<br />

4.4. Discussions …………………………………………………………………...…82<br />

4.4.1. Body size, fecundity and egg size ……………………………………………….……82<br />

4.4.2. Depth allocation <strong>of</strong> the species and possible causes ……………………..……86<br />

CHAPTER FIVE – SYNTHESIS AND CONCLUSIONS<br />

5.1- Factors controlling the bathymetric distribution <strong>of</strong> species and their effects on early<br />

life-history stages <strong>of</strong> echinoderms<br />

…………………………………………………….94<br />

6

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