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X Data
blastulae and ~50 % abnormal, at 150 atm ~60 % were abnormal, ~30 % blastulae and
~10 % uncleaved, at 200atm ~90% were abnormal, the rest were uncleaved. At 20°C
at 1atm ~70 % of embryos were early gastrula, the rest were abnormal, at 50atm ~70
% were early gastrula, the rest were abnormal, at 100atm ~90 % were abnormal, the
rest were blastulae, at 150 atm ~70 % were abnormal,~ 30 % were early gastrula, at
200atm 70 % were abnormal, 16% were blastula and 14% were uncleaved (Fig. 3.14).
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5 o C
10 o C
15 o C
U 2 4 8 32 BA U 2 4 8 32 BA U 2 4 8 32 BA
D evelopm ental Stage
20 o C
U 4 8 3264 BEgA
200 atm 150 atm 100 atm 50 atm 1 atm
Fig. 3.14. Marthasterias glacialis embryos incubated at 5, 10, 15 and 20° C at 1, 50,
100, 150 and 200 atm for 24 h. Development stages are (U) Uncleaved, 2 to 32 cell,
(B) Blastula, (Eg) Early gastrula and (A) Abnormal.
At 48 h: At 5°C and 1 and 50atm more than 90 % of embryos were abnormal
the rest were uncleaved, at 100 and 150atm ~90 % of embryos remained uncleaved,
the rest were abnormal, at 200 atm ~80 % underwent abnormal cleavage and the rest
remained uncleaved. At 10°C and 1 and 50 atm ~90 % of embryos were blastula, the
rest were abnormal, at 100atm ~60 % of embryos were abnormal, the rest were
blastulae and uncleaved, at 150atm all the embryos underwent an abnormal cleavage,
at 200atm 60% had abnormal cleavage and 40% were uncleaved. At 15°C and 1 and
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