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Download (3398Kb) - ePrints Soton - University of Southampton

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traits were obtained in the laboratory from fixed specimens archived in the Discovery<br />

Collections at the National Oceanography Centre, <strong>Southampton</strong> (NOC). This material<br />

was collected at depths from 150 to 4850 m. The samples were obtained over a period<br />

<strong>of</strong> 20 years as part <strong>of</strong> different programmes. The samples were collected using a semiballoon<br />

otter trawl (OTSB14) and a Granton trawl.<br />

The specimens were first fixed in 10% formalin, and transferred to 70%<br />

alcohol for storage.<br />

Fig. 4.1. Bathymetric chart <strong>of</strong> the Porcupine Abyssal Plain (PAP) and Porcupine<br />

Seabight (PS), NE Atlantic (Image modified from GEBCO 97).<br />

The gonads <strong>of</strong> 5-10 specimens per sample were dissected out by opening the<br />

oral side <strong>of</strong> the disc along each radius, previously the whole animals had been dampdried<br />

and weighed. The major radius (R), from the centre <strong>of</strong> the disc to the tip <strong>of</strong> the<br />

70

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