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were abnormal. At 150 and 200atm all the embryos were abnormal. At 15°C and from
1 to 150atm nearly 50 % of the embryos were blastulae, the number of abnormal
embryos was increasing with pressure from ~ 20 % at 1atm to ~50 % at 150 atm, at
200 atm all embryos were abnormal. At 20°C all embryos were abnormal (Fig. 3.9).
percentage
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5 o C
10 o C
15 o C
U 2 4 8 32 BA U 2 4 8 32 BA U 2 4 8 32 BA
20 o C
U 2 4 8 32 BA
200 atm 150 atm 100 atm 50 atm 1 atm
D ev elo p m en tal S tag e
Fig. 3.9. Asterias rubens embryos incubated at 5, 10, 15 and 20° C at 1, 50, 100, 150
and 200 atm for 12 h. Histogram represent % mean and SD. Development stages are
(U) Uncleaved, 2 to 32 cell, (B) Blastula and (A) Abnormal.
At 24 h: At 5° C and 1atm ~80 % of the embryos were 64-cell and the rest
were abnormal, at 50 and 100 atm ~ 80 % were abnormal, the rest remained
uncleaved, at 150atm almost 90 % of the zygotes were uncleaved, the rest were
abnormal,at 200atm all the embryos were abnormal. At 10°C and 1atm between 60
and 70 % of the embryos were blastulae, ~15 % were 64-cell or abnormal equally, at
50atm ~50 % of the embryos were blastulae, the rest were abnormal, at 100atm ~80 %
were abnormal and the rest were blastulae, at 150 and 200atm all the embryos were
abnormal. At 15°C from 1 to 100atm the number blastulae was decreasing from ~80
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