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X Data
to ~50 %, the rest were abnormal, at 150atm ~60 % were abnormal, the rest were
blastulae, at 200atm all the embryos were abnormal. At 20°C at 1atm >95% of
embryos were abnormal and the rest were blastulae, from 50 to 200atm all the
embryos were abnormal (Fig. 3.10).
percentage
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5 o C
10 o C
15 o C
U 2 4 8 32 BA U 2 4 8 32 BA U 2 4 8 32 BA
20 o C
U 2 4 8 32 BA
200 atm 150 atm 100 atm 50 atm 1 atm
D evelopm ental Stage
Fig. 3.10. Asterias rubens embryos incubated at 5, 10, 15 and 20° C at 1, 50, 100, 150
and 200 atm for 24 h. Histogram represent % mean and SD. Development stages are
(U) Uncleaved, 2 to 64 cell, (B) Blastula and (A) Abnormal.
At 48 h: At 5°C and 1atm ~50 % of the embryos were blastulae, the rest were
abnormal, from 50 to 150atm almost 90 % of them were abnormal, the rest remained
uncleaved, at 200atm all embryos were abnormal. At 10°C and 1atm ~60 % of the
embryos reached the late gastrulae stage, the rest were abnormal, at 50 and 100atm
~10 % were gastrulae and the rest abnormal, at 150 and 200atm all embryos were
abnormal. At 15°C and 1atm almost 80 % of the embryos were late gastrula, the
remainder were abnormal, from 50 to 150atm the number of abnormal embryos was
~70 %, the rest were late gastrulae, at 200atm all the zygotes were abnormal. At 20°C
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