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Universlty of Manitoba, ln Partîal Fulfiìlment - MSpace at the ...

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Ro<br />

Comparison <strong>of</strong> Figs. 12, lJ and 14 shows thät obliter<strong>at</strong>ion <strong>of</strong> <strong>the</strong><br />

introduced air space reduces its ter<strong>at</strong>ogenic effect, part¡cularly when<br />

performed immedi<strong>at</strong>ely after windowíng. comparlson <strong>of</strong> <strong>the</strong> st<strong>at</strong>isticêl<br />

analyses, however, shows some incons¡stencies between <strong>the</strong> three<br />

expe r¡ ments 3<br />

(") early de<strong>at</strong>hs and deveroping embryos in <strong>the</strong> combined experimental<br />

groups and <strong>the</strong> control group:<br />

albumen ¡ n troduct ¡on<br />

F12 înt roduct i on<br />

air cell reexpanslon<br />

N.S.<br />

N.S.<br />

P < 0.01<br />

(b) early deêths and developing embryos in each experimental group:<br />

albumen introduction<br />

F12 i nt roduct i on<br />

aír cell reexpansion<br />

N.S.<br />

N.S.<br />

P < 0.01<br />

(") de<strong>at</strong>hs and defects in developíng embryos <strong>of</strong> each developing group<br />

<strong>of</strong> experimental emb ryos<br />

albumen ¡ntroduction<br />

F12 introduction<br />

air cel.l reexpansion<br />

P < 0.01<br />

N.S.<br />

0bl iter<strong>at</strong>ion <strong>of</strong> <strong>the</strong> introduced air space thus reduces <strong>the</strong> early<br />

deãth r<strong>at</strong>es within and between <strong>the</strong> experímental groups when albumen or<br />

F12 (but not air cell reexpansion) are emproyed. A more subtre reduction<br />

<strong>ln</strong> l<strong>at</strong>er de<strong>at</strong>hs and malform<strong>at</strong>íons is achieved by air cerr reexpansion<br />

(but not by albumen or Fl2), Despite <strong>the</strong>se difference <strong>the</strong> experiments<br />

confirm th<strong>at</strong> <strong>the</strong> presencè <strong>of</strong> an artìf ical. air space above.<br />

chick embryos <strong>at</strong> early developmental stages is hlghly ter<strong>at</strong>ogenic.

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