Universlty of Manitoba, ln Partîal Fulfiìlment - MSpace at the ...

27
lf necessary, eggs were stored at 10oC for up to four days before use.
The two incubators used were both suppl ìed by the Blue M Electric
company (Blue lsland, ll l.). The larger convection model (2004) measured
48 cm. x f6 cn. x 4! cm.,and contained a 15 cm. x lB cm. x 5 cm. dísh of
water for regulation of humidîty. lt was used maînly for reincubatlon
of eggs after some form of treatment, especially in experiments involving
prolonged culture. Satisfactory regulation of temperature (37.50 t loC)
and humidity(60?. t 4%) was achieved.
The smal ler forced-draught model (VP-1004T-1) consigted of a 42 cm.'
x \2 cn. x 30 cm. plexiglass contaìner above a humidifier, wlth a fan
provídîng a continuous airflow at wel l-control led tenperature (37.50 t
0.5oc) and humidity (60Z ! 1Zl. lt was used mainly for short term
exper¡ments and for the ¡nitial incubat¡on of eggs before treatment
(Fis. 5).
The work descrîbed in thîs thesîs was performed ín a single room
facing south, with large windows and no air-condítioning. Regulation
of heating and ventilation gave partial control of the ambient env¡ronmentrand
the two incubators used provided stable incubation conditions.
Exper¡ments were performed throughout the yeâr.
J.3 OTHER EQUIPI4ENT
All operations were performed under steríle or semi-steriÌá
conditions in a dust-free plexiglass cabinet cleaned with 70% alcohol
before each exper iment.
stainless steel instruments, such as forceps, were steril ized
before use and passed through the flame of an alcohol burner several
tlmes during an operat ¡ on.