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Universlty of Manitoba, ln Partîal Fulfiìlment - MSpace at the ...

Universlty of Manitoba, ln Partîal Fulfiìlment - MSpace at the ...

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67<br />

5.3 INVFSïIGATtON 0F THE TERAToGENTC EFFECT:0F t;/lNpor,ilNG<br />

<strong>ln</strong> an <strong>at</strong>tempt to define some <strong>of</strong> <strong>the</strong> factors leading to abnorrnal<br />

development after windowing, several experiments were performed to<br />

investig<strong>at</strong>e varlous aspects <strong>of</strong> <strong>the</strong> windowi.ng technic, <strong>ln</strong> one group <strong>of</strong><br />

experiments eggs were trear:ed bv vîbr<strong>at</strong>ion, <strong>of</strong> by <strong>the</strong> applic<strong>at</strong>íon <strong>of</strong><br />

parafî lm and a plasticine iing, wÌthout windowîng. <strong>ln</strong> o<strong>the</strong>r experiments<br />

<strong>the</strong> ¡ntroduced air space was obl iter<strong>at</strong>ed <strong>at</strong> <strong>the</strong> time <strong>of</strong> windowing.<br />

5.3.1 Vibf¡itiori óf UnoÞened Eggs,<br />

,.m;r<strong>at</strong>ÎonProducedby<strong>the</strong>dentalsandIngdiscusedto<br />

cut <strong>the</strong> shell w<strong>ln</strong>dows, unopened eggs were vlbr<strong>at</strong>ed for J0 seconds wlth a<br />

carborundum ball mounted on <strong>the</strong> dr¡ll. Thls procedure was performed ât<br />

0, 26 and 33 hours <strong>of</strong> încub<strong>at</strong>lon, and <strong>the</strong> eggs <strong>the</strong>n re<strong>ln</strong>cub<strong>at</strong>ed to a total<br />

<strong>of</strong> / days without be<strong>ln</strong>g turned.<br />

Tables 10 and tt show <strong>the</strong> mortal ìty and malform<strong>at</strong>ion r<strong>at</strong>es for <strong>the</strong><br />

three experîmental groups and <strong>the</strong> controls. Bêcause <strong>of</strong> <strong>the</strong> rel<strong>at</strong>ively<br />

small numbers ín each group, values are not converted to percentages.<br />

Desplte <strong>the</strong> small numbers ît is clear thðt V¡br<strong>at</strong>¡on by itself is not<br />

responsîble for <strong>the</strong> ter<strong>at</strong>ogenic effect <strong>of</strong> <strong>the</strong> windowíng techníc. There<br />

werè no open èord defects in any <strong>of</strong> <strong>the</strong> experimentêl or control groups.

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