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63 5.2 MALFoRMAT I 0NS .P¡OpUCEp BY \ir l ND0Vill NG . Analysîs of the nalformatlons produced by the windowing technic required culture of treated embryos to the perìod when the external embryonic form had been establ lshed. By this time, however, the mortal ity among developing embryos was substantial, and many of the dead embryos were so necrotlc that full examìnatlon was impossible. For thîs reason, embryos wlndowed at 26 hours and developing to 5 days or 12 days were compared to the 72 hour (3 days) group in Section 5.1. - Rs ihis was an analysis of the external malformatîons produced by the wlndowing technlc, it includetl only well-preserved experimental embryos showing con'tinued dèvelopment after windowìng. Table 9 and Fi9.11 show the external defects recorded at 3,5 and 12 days of incubation, expressed âs percentages of those developlng embryos which could be examined fully. These values do not represent the ou..ull malformadion rates in treated embryos but, rather, reflect the changing pattern of malformations wi th prolonged g rowth . ln Fi9. Il the most striking finding is the uniformly high incidence of open cord defects in all three groups. 0f the non-neural ma I fo rma t,îions , def ects învolving the face-and beak, trunk, rump and ta¡1, anterior body wall,and lower I imbs appear with íncreasing frequency at progressively later stages of development.
TABLE 9. ¡4ALFORMATIONS PRODUCED'BY [TINDO\iING'AT'26'HOURS ?:?:v: {ll::: ¡ ?ly: {Tì l2 Days (Z) Developlng Emb ryos 53 109 69 open Cord Defects 25&7.12') ç3 (57 .8) 41(59.42) 0pen Bra ln Defects 2(3,77) 4 (3.671 6 (8.70) Ml crocepha I y 14(26.\z',) 1o (9. r 7) 6 (8.70) Eye Defects \0.55) \2(38.53) 19Q7.5\) Face E Beak Defects 0 (0) 1l (10.09) 21(30.43',) Trunk Defects 6(11.32) 8 (7.34) lo(14.49) ' Rump e Tail Defects 0 (0) 30(27.521 31(44.93) Ectopia V I sce rum 0 (0) 0 (0) 27 ß9.13) Lowe r Llmb Defects 0 (0) \ß.67',) 17 Q\.6\) Upper Llrnb Defects 0 (0) 1(0.92) 0 (0)
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E}4BRYOGENES IS OF EXPERIHENTALLY I
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MY PARENTS c.J.H. G. M.
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lt Congenital malformêtlons of the
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TABLE OF CONTENTS SECT ION PAGE 1 I
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},I.ATE R I ALS 3.1 THE CHICK EI4BR
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vt I I 6.3.2 Stage 10 Experímentel
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9 APPENDTCES 378 APPENDIX A . 379 1
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1.1 TERATOLOGY Birth defects have a
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4 of the complexity of normal devel
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6 flat, exposed plaque of neural ti
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8 However, several early embryos in
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.1- 5
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l2 2.1 ErI0L0rL!! fïE qYs34t!1!!jr
Page 28 and 29: 4 which has rema¡ned fairly consta
Page 30 and 31: 6 and about 112 after two (Laure,nc
Page 32 and 33: l8 ln some very early human embryos
Page 35 and 36: 2l and kinky-ta¡l as disorders of
Page 37 and 38: 23 Vogel and McClenahan (1952) tnus
Page 39 and 40: I'IATE R IALS 25
Page 41 and 42: 27 lf necessary, eggs were stored a
Page 43: FÍg. 5. lncubator allowing precise
Page 46 and 47: 3l 4. r sELEcr I oN !F EGGq 4.1,1 .
Page 48 and 49: uler. Accurate Staging (Hamburger a
Page 50 and 51: .\lz '?. t
Page 52 and 53: Fis. I AsB, Windowìng followed by
Page 55 and 56: Fis. 9 Aã8. t/indow?ng followed by
Page 57 and 58: 42 ( \--l /) ^ ) t0
Page 59 and 60: 44 serîal sectlonlng a camera luci
Page 61 and 62: 46 Thus the embryos selected for se
Page 63 and 64: 48 TABLE 3. srAGE t0 El,tBRyos (cno
Page 65 and 66: 50 TABLE 5 , Reg i ons srAGE 13:16'
Page 67 and 68: 52 4.8.4 HistologÌcal_De:plþtÍon
Page 69 and 70: 5 RESULTS OF TERAÎOLOGICAL PROCEDU
Page 71 and 72: (c) deaths and defects in the survi
Page 73 and 74: Fi g. Percentagcs of early deaths a
Page 75 and 76: 6o MoRTALtTY, AFTER ì,'tNpowtNq,AT
Page 77: TABLE B.'DEVEIOPI{ENT'AFTER'WINDOIi
Page 81 and 82: N=231 ffil e ooyi ffi s oays [il t2
Page 83 and 84: 6B TABLEt loj lloRTALtTy AFTER VtBR
Page 85 and 86: 70 5,3.2 Pa-rôf i Idì ¿irid PIè
Page 87 and 88: TABLE 13; MALFORI4ATIONS AFTER PLAS
Page 89 and 90: 41 13 0 23 No l^l i ndow Control s
Page 91 and 92: Fíg. Percentages of early deaths a
Page 93 and 94: 78 ALBUMEN INTRODUCTION Analysís o
Page 95 and 96: 0 1 0 0 a o TABLE 17. I'lALF0RÌ1AT
Page 97 and 98: N Eorly Deothi N=10ó N a ú, o d,
Page 99 and 100: 103 51 52 28 14 4 6 No W î ndow Co
Page 101 and 102: Fi g. 14. Percentages of early deat
Page 103 and 104: 88 AtR qELL, REEXPANSI0N Analysls o
Page 105 and 106: 90 5.4 BACTERt4L CUTTURE 0F UlNpohr
Page 107 and 108: 92 TABLE 22. ¡¡UI'ISELTS OF PLATE
Page 109 and 110: 94 6.1 EMBRY0ûIIES I s 0L 0!!\- NE
Page 111 and 112: Fis. I5 A¿8, Range of s izes (mm.)
Page 113 and 114: Figs. 16 AaB.. Range of sizes (mm.
Page 115 and 116: 100 6.1.2 f4ortal í!y with Vsrying
Page 117 and 118: 421.. 26 25 I (3.85) 20 (76 .92) 1
Page 119 and 120: 00 N Eorly Deoths ,n -¡ F- ô- xu.
Page 121 and 122: ì06 Degrees of Freedom 12 Chi Squa
Page 123 and 124: hzE 53 42C z5 51(96.4)2\(96) 2(3.77
Page 125 and 126: ffil Op"n Anterior Neuropore I I0 N
Page 127 and 128: N=380 El Ovol Rhonnboid 'Sinus ì l
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Øl Open Broin Defecls N=384 ffi F"
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ligr. 21 - 30. Camera lucida drawin
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Fi s. 22. lrregular open anteríor
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lmm OPEN BRAIN DEFECT Á,NOPHTHALMI
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lmm OVAL RHOMBOID S¡NUS 48 HOURS S
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FiS. 26, Open neural folds extendin
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t-l lmm IATER OPEN CORD DEFECT . RE
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l2B t--l lmm EARLY OPEN CORD DEFECT
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Fis. 30. Large caudal cyst Ìn an o
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132 6.1 .4 DeveloÞñent of oÞen N
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Fig. 31 . Percentêges of experinen
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36 TABLE ?8. RH0I4B0lD stNUs ctôs
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N=38o H ovol Rhomboid sinus l^ r38
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F¡ S. 33. Percentages Õf open cor
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142 6.1.5 Distríbution of 0pen Cor
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Fi s. "l! Percentage distrìbution
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lrregul ar (Z) somi te be low level
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ì48 N=2ó4 ffi m Regulor Cord Defe
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Fis. 36. Percentage distrîbution o
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152 6.2 sPtIA! LFVELS 0F oPEN CORp
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Fìgs. 37'38, Vertebral defecrs in
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CERVICAT THORACIC LUMBAR FUSED CAUD
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---l r r'I * 40
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t59 a) spîna bif¡da occulta is se
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:al . '**Ç- 4 ff, À(,) ftø 9-' o
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162 lmnediately after closure, sepa
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164 Hensenrs node and the primitive
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-l :: l 48 I 49 i :. t: .lì 50 ì:
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t67 somite area (through asymmetry
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t69 ln both embryos the volume of n
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171 The rhomblc roof became membran
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173 At areas of myeloschisîs conta
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175 neurêl tîssue wíth mesoderm
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t77 e) encroachment of somitîc mes
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ôt rf) , ,,. ì _ ^iiir¡:ù\. I r
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l 55 56 57 58 59 ó0
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Fiss. 65 Early myeloschisis in a St
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fiSr. 71 - 76. Later myeloschisis i
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Fiss. 77 -82. Early myelodysplesia
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FÌgs. B3 - BB. Later myelodysplas
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Figs, 89 - 91. Processíng artifact
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ì86 6.3.10 SequenJ¡¿il ll lúSti
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Fígs. 95 - 102, Sequentiêl drawin
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l9o EVERsToN: srAGE rf (o r o+) o @
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@ @@ "@ @ e q q a a 192 CoNTROL: ST
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MYELOSCHTSTS: STAGE tZ (tZeïl OOG
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coNTRO[: STAGE tó (ro c zz) . a@ Q
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G (õ--==: \z-- HEMTMYELIA: STAGE t
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Som i te Defects contact none sePa
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Somite Som i te Contact Defects N'
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Som i te Contact Som i te Defects c
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none none N) o o\ B r8c 23 12 good
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separat ion none contact/ none sepa
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Regíon Embryo Stage Conditîon Neu
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none none none none none none l'.J
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18E 10 13- vëry poor closed 0 cove
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separãtion none contact none conta
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Regíon Embryo Stage Condition Neur
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separation none sepâ ra t îon non
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separâtion none sepa ra t ion sepa
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Reglon Embryo Stagd cond¡t¡on Neu
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none none none none N) NJ .{ A A A
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contact/ none separatlon contact no
Page 256 and 257:
Region Embryo Stage Conditlon Neura
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TABLE 38D. STAOE 17.20 CONTROL AND
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Reglon Embryo Stage Condition Neura
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428 72 20 closed covered none conta
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239 neural tissue ât the later sta
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241 The control group (Table 99 ) s
Page 268 and 269:
TABLE 39. APPEARANCE OF I,'HOLE EI4
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TABLE 40. APPEARANCE OF }/HOLE EI"I
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closed closed c I osed c I osed c I
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249 6.6 pEVEL0PMENT 0F rH!_¡Ho|4gl
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Emb ryo Stage Rhombîc Roóf Neural
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CDE D'E DE CDE DE DE DE D BCD BC BC
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TABLE q6,. STAGE 17-20 CoNTRoI AND
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Figs- 10J - i11. Development of the
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Fî s. tub. Thick rhombic roof in 5
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Fig. 109 Hernbranous rhombic roof w
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260 6.7 HrqroLoctcAL, cHANgË Assoc
Page 290 and 291:
TABLE 47. CONTROL EMBRYoS.HISTOLOGI
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0 0 0 0 0 0 0 0 0 0 o 0 0 0 0 0 0 N
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42c 21 zo B c D E 0000 00 00 00 1\.
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l\t q\ 1oE 26 16 30E 59 16 3oE 77 1
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0 0 0 0 0 0 0 0 0 0 0 0 N) .{ Embry
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l\) \l N D E 00 00 0 0 13.79 0 100
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N) \¡ \2E 72 20 E B c D' E 00 0 15
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46. 15 76.92 0 20.51 14.83 2i,92 53
Page 306 and 307:
ECTODERM DISCONTINUITY IN EXPERIMEN
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279 ECTODERM DISCONTINUITY IN EXPER
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SOMITE SEPARATION IN EXPERIMENTAL E
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283 NOTOCHORD SEPARATION IN EXPERIM
Page 314 and 315:
285 SOMITE DEFECTS IN EXPERIMENTAL
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287 and distr¡bfrtíon in the cont
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19.09 r8.95 r 1.84 14.82 15.20 0 17
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0 15. 89 81 .08 100 100 ' 76.92 0 1
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43. rB 100 100 0 12.53 74.07 r00 't
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7.t6 16.03 100 100 160 7.90 18.22 r
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Figs. 120'123. Percentage ie.ngths
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299 O\TERLAP ZONE IN EXPERIMENTAL E
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301 OVERLAP ZONE IN EXPERIMENTAL EM
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303 ând every tenth sectlon was ne
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30c 12 16 c 17707,61 2463.3\ 7.19 D
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Ratio NT/N 4 .90 4.11 6.17 7.14 6.9
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Rêtlo NT/N 4.go \.36 3 .44 3. 18 3
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6.7t 6.lt 4.63 4.60 4.13 3.48 hzE 5
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Fi9s, 124-127, Neurál tube-norocho
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315 NEURAL TUBE -NOTOCHORD RATIOS I
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317 NEURAL TUBE-NOTOCHORD RATIOS IN
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319 TABLE 598 ' ANALYSIS OF VARIANC
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321 TABLE 61., .NEURAL TISSUE/NOTOC
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323 Apart f.rom the sîgnîficant d
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t25 7. Dlscusst0N 1'/i th progressi
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327 C¡osure of the anterior neurop
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329 the mid-polnts of the defects w
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331 'c¡osure of the neural folds w
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333 . These findings s.ugges r that
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335 Lendon (1968, 1975) and notos (
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337 Ferm, 1965; l"larin-Padi r ¡a,
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339 Kôplan (1965) and Kaplan and G
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341 reach the floor-plate; asymmetr
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343 took up the label, shovri.ng th
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345 The defects in early human embr
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347 after pred¡cted closure (Lemî
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349 hours could provide evidence fo
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351 t971), Gal lus and Xenopus (l'l
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353 1962i 1965). Barth and Barth.(1
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The result of any teratogen¡c insu
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357 Uindowîlg at ear¡y stages of
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SUHHARY AND CONCLUS I ONS
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361 separatlon between the neural p
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363 APPENDIX A Preparatlon of Early
Page 394 and 395:
365 APPENDIX B Stainl.ng of Carti l
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367 10, BIBLIOGRAPHY, Alter, l.,l.
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369 Braun, l'{. (1882). entwicklung
Page 400 and 401:
371 Crlley, B,B.(1969). Analysîs o
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373 dqs anomal ies de lrorganizatio
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375 Heath, H.D., Shear, H.H., lm€
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377 Jel inek, R. (1960). Developmen
Page 408 and 409:
379 Laurence, K.14. (1964). Thé na
Page 410 and 411:
381 McKeown, T. and Record, R.G.(19
Page 412 and 413:
Penrose, L. S.(1957). Genetics of a
Page 414 and 415:
385 Russell, H.E. and A¡ tken' G.T
Page 416 and 417:
387 Spemann, H. (1938). Embryonic D
Page 418 and 419:
389 lJeed, ¡.H. (1937 -38'). M.nin
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