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3i<br />
Two of the 6 x 2 m. tanks<br />
four experimental groups vere<br />
weie divided longitudinally vith a<br />
placed inside.<br />
srid and the -<br />
Treatment duration for all<br />
weight and length control {as<br />
experirnental<br />
KepE.<br />
groups lras 8 nonths.<br />
A<br />
binonthLy<br />
2.2. Diet Adroinistration<br />
lte ingledients<br />
uooden table in the<br />
(4pro..).<br />
were mixed and placed<br />
dry zone of the tank.<br />
in approximate 2 cm. pieces on a -<br />
The diet was administered once a day<br />
2.3. Weisht<br />
The babas were veighed every 2 nonths per group on a 20 Kg. capacity -<br />
scale (Jacobs Manvill),<br />
2.4. SIze<br />
The babas were neasured everv 2 nonths individuallv from tail to snout<br />
2.5. Histopathologlcal Study<br />
Du.ing the first two nonths of treatment, nortality in aII babas<br />
(fundanentalty in the control group) vas vely high, thus 10 controf group<br />
specimens !?ete sacrificed by cervical dislocation and henatotogical and parasitological<br />
exaninations lrere peiformed (Marine Biologist, Gina Annas de Conroy,<br />
M. Sc. Aguatic Pathobiologist ) , These examinations were car:ried out by -<br />
observing fresh pr:eparations of the different organs and annexes, such as -<br />
1ungs, 1iver, spleen, intestine, stomach, trachea, oesophagus and blood.<br />
Blood sanples were extracted by cardiac puncture. Sodiun heparine !/as<br />
used as an anticoagulant. Blood snears uere, colored llith GIB,ISA.<br />
Once all treatments lueie finalized (8<br />
neasur:ed (Iength and r.idth) and the sex<br />
works of (Chabreck, 1967; and Brazaitis,<br />
fe.na1 specitnens was obtained.<br />
nDnths), the babas lrere veighed,<br />
was deteranined according to the<br />
1969). The sane amount of male and<br />
3.. RESULTS<br />
3,1. The effect of diets<br />
flour and a complemented<br />
conplemented with sodium L-Thyroxine, white<br />
of vitanins and essential anino acids on: