Presidential Greeting - American Society for Laser Medicine and ...

2 American Society for Laser Medicine and Surgery Abstracts
signing that LLLT can significantly contribute to prevent such
appalling condition.
#3
NOVEL APPROACH FOR HEALING LEG ULCERS:
CASE STUDY INVOLVING COMBINED EFFECT OF
LOW LEVEL LASER THERAPY AND
BIOCERAMICS
Supriya Babu, Bheemsain Rao, Veena P. Waiker,
Jaisri Goturu, Vasanthi Ananthakrishnan,
Dayananda G, Arun Kumar, M.S. Ramaiah
Institute of Technology, Bangalore, India; M.S. Ramaiah Medical
College & Hospital, Bangalore, India
Background: Of all the wounds, leg ulcers are more common in
Indian population and are a major cause of amputation. An
increased resistance of micro-organisms in the wound calls for
novel approaches in wound management and therapy. We propose
combining the bacteriostatic action of Bioceramics and the wellestablished
advantages of LLLT in achieving a quick recovery of
the ulcer with reduced treatment cost.
Study: A male patient aged 60 years with chronic non-healing
venous ulcers on both lower limbs. The lateral half of the both the
wounds was used as control with routine dressings with betadine.
The medial half of the right leg ulcer was treated with bioceramic
wound healing device (WHD), The medial half of left leg ulcer was
treated with LLLT (0.63 mm, 10 mW, CW) for 10 minutes and later
dressed with WHD. Treatment was given for seven consecutive
days and serial photographs were taken. Efficacy was assessed by
taking bacterial culture and sensitivity (C/S) on first and eighth
day.
Results: Significant improvement was noticed with respect to the
exudate amount, color, and edges in ulcer treated with combined
therapy whereas minimal change was seen in WHD therapy
alone. No significant changes were seen on control side of the
wound. The initial C/S report indicated bacterial growth and in
the final report, no growth of organisms was noted.
Conclusion: With the promising effects of combination therapy
for chronic, non-healing venous leg ulcers requires further
controlled studies with larger study group to validate the results.
#4
EFFECT OF POLYETHYLENE GLYCOL COATING
ON BIODISTRIBUTION OF ICG-LOADED
POLYMERIC NANOCAPSULES IN MICE
Baharak Bahmani, Sharad Gupta, Bahman Anvari
University of California, Riverside, CA
Background: Nano-constructs with near infrared (NIR)
absorption capability present a promising technology for optical
imaging and phototherapy of malformations. The advantage of
utilizing NIR light is minimum absorption by water and tissue
components and consequently higher penetration depth.
Indocyanine green (ICG) is the only FDA approved near infrared
chromophore used clinically in imaging applications, and is under
investigation for photothermal and photodynamic therapy.
However, ICG suffers from short circulation time within the
vasculature, and is almost exclusively uptaken by the liver. To
overcome these shortcomings, we have encapsulated ICG within
polymeric nanocapsules (ICG-NCs). Our long-term objective is to
enhance circulation time of ICG through nano-encapsulation, and
use the encapsulated platform for site-targeted optical imaging
and phototherapy. Here, we investigate if surface coating using
polyethylene glycol (PEG) will alter the biodistribution of ICG-
NCs.
Study: We synthesize ICG-NCs using a self-assembly process
that utilizes polyallylamine hydrochloride and sodium phosphate
salt as the encapsulating structure. ICG-NCs are coated
covalently with PEG of either 5,000 or 30,000 Da molecular
weight, through reductive amination. PEG-coated ICG-NCs are
administrated through tail vein injection in healthy mice. Whole
body florescent imaging is performed at different post-injection
times. To quantify the biodistribution of PEG-coated ICG-NCs,
various organs including liver, lungs, spleen, intestine, kidneys
and heart are harvested and homogenized following euthanasia.
Blood sample is also collected to investigate amount of PEGcoated
ICG-NCs remaining in the vasculature at different postinjection
times.
Results: Our preliminary results suggest that encapsulation of
ICG in polymeric nanocapsules alters the dynamic biodistribution
of ICG in healthy mice. PEGylation of ICG-NCs appears to delay
the accumulation of ICG within the liver in comparison with
uncoated ICG-NCs and free ICG.
Conclusion: Results of this study will provide important
information in engineering ICG-NCs with prolonged blood
circulation time and potential applications for fluorescence
imaging and phototherapy of various abnormalities.
#5
NARROWBAND IMAGING OF TARGETED GOLD
NANORODS IN TUMORS
Priyaveena Puvanakrishnan, Parameswaran
Diagaradjane, Glenn Goodrich, Jon Schwartz, Sunil
Krishnan, James Tunnell
University of Texas at Austin, Austin, TX; University of Texas/MD
Anderson Cancer Center, Houston, TX; Nanospectra Biosciences,
Inc., Houston, TX
Background: A significant challenge in the surgical resection of
tumors is accurate identification of tumor margins. Current
methods for margin detection are time-intensive and often result
in incomplete tumor excision and recurrence of disease. Gold
nanoparticles have recently gained significant traction as
exogenous contrast agents for identifying tumors ex vivo. The
objective of this study was to determine the potential of topically
administered antibody conjugated gold nanorods (GNR) for realtime
tumor margin detection using near-infrared narrowband
imaging (NIRNBI). NIRNBI images narrow wavelength bands to
enhance contrast from plasmonic particles in a widefield, portable
and non-contact device that is clinically compatible for real-time
tumor margin demarcation.
Study: We conjugated GNR to Cetuximab, a clinically approved
humanized antibody that targets the epidermal growth factor
receptor (EGFR). We excised subcutaneous xenografts of
squamous cell carcinomas from Swiss nu/nu mice and divided the
tumors into two groups: (1) the targeted group (anti-EGFR
conjugated GNR) and (2) the control group (PEG-conjugated
GNR). After topical application of particles and incubation for
30 minutes, the tumors were washed and imaged using NIRNBI.
To quantify the binding of GNR in tumors, we measured the
contrast enhancement for each particle type.
Results: The NIRNBI images showed a visual increase in
contrast from tumors administered with targeted GNR over the
control particles and without the particles. There was a
statistically significant increase in contrast (400%) from tumors