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78 American Society for Laser Medicine and Surgery Abstracts
#262
Study: This study was conducted on 18 patients with gingival
hyperplasia, randomly divided into two study groups, group I
(laser) and group II (conventional). The laser device used in group
I was represented by an Er:YAG laser (2,940 nm, LP, VLP, 140–
250 mJ, 10–20 Hz). In group II, the gingivectomy was performed
with a microsurgical scalpel. Gingival microcirculation was
recorded using two Laser Doppler Flowmetry channels available
for simultaneous measurements of two sites, before, 1 week after
treatment, 1 and 6 months post-treatment. All collected data were
processed and analyzed statistically.
Results: The collected data showed significant increase of
gingival blood flow 1 week after treatment as compared to the
baseline (P < 0.005). After subsequent examinations, the gingival
blood flow was similar to the baseline (P > 0.005). Moreover, in
group I, the blood was similar to the baseline at 15 days and at 30
in group II (P < 0.005).
Conclusion: Under the study conditions, laser-assisted
treatment proved to be more effective than conventional
approach. The results of this study demonstrated that Laser
Doppler Flowmetry can be useful in the management of healing
process.
#261
ROOT CANAL MICROLEAKAGE INVESTIGATION
AFTER ER:YAG LASER-ASSISTED TREATMENT
Cosmin Balabuc, Laura Filip, Aurel Raduta,
Cosmin Locovei, Carmen Todea
University of Medicine and Pharmacy of Timisoara;
Politehnica University of Timisoara, Timisoara, Romania
Background: In endodontics, the quality of the root canal filling
is the result of the root canal cleaning and shaping, leading to the
prevention of leakage. The aim of this study was to investigate
using Scanning Electron Microscopy the effect of Er:YAG laser
irradiation of the root canal in reducing the microleakage.
Study: Twenty-four extracted teeth with one straight root canal,
closed apices and extracted for periodontal reasons were used in
this study. The crowns were removed by transversal sectioning
and the roots were submitted to biomechanical treatment. After
the biomechanical treatment, the teeth were divided randomly
into two study groups, group I (laser) and group II (conventional—
control), each of 12 teeth. The teeth from group I received laser
irradiation of the root canal. The laser device used was
represented by an Er:YAG laser (VSP, 40–80 mJ, 20 Hz). The
teeth from group II received only conventional biomechanical
treatment. Then, all the root canals were dried with paper points
and filled with root canal sealer in conjuction with gutta-percha
points using lateral condensation and vertical compactation
techniques. Then, the roots were embedded in acrylic resin using
specially designed conformators and 5 mm sections were
performed from apical to coronal direction of the root. The voids
inside the root canals were quantified and the measurements were
analyzed statistically.
Results: The investigation evidenced the presence of voids inside
the root canal, microleakage areas being detected between the
root canal sealer and the dentine root canal wall, but also at the
gutta-percha – sealer interface. Most of the defects were noticed
in the group were only conventional biomechanical preparation of
root canal was performed.
Conclusion: The results of this study demonstrated a reduction
of microleakage in the apical area when using Er:YAG laser
irradiation in combination with the conventional biomechanical
root canal preparation.
MELASMA TREATMENT WITH A COMBINATION
OF TOPICAL CREAMS AND PULSED CO2
FRACTIONAL ABLATIVE RESURFACING
Mario Trelles, Mariano Velez
Instituto Medico Vilafortuny, Cambrils, Spain
Background: Melasma is difficult entity to treat and, in general,
antipigment cream is the treatment of choice but with inconstant
results. Fractional lasers have been proposed as a useful
treatment. This presentation reports on treatment with topical
creams combined with a pulsed CO2 ablative fractional
resurfacing.
Study: Twenty-two females, suffering from melasma, treated
with an antipigment cream program following pulsed CO2
ablative fractional resurfacing with a follow-up of 2 years, are
presented. CO2 laser fixed pulse width, high power, low frequency
non-sequential pulses were used first. Then, an antipigment
cream at low dosage to be used regularly every day and once the
skin was free of scabs. Patients, mean age was 36 years with skin
types II–IV. Subjective patient and clinician assessments
according to melasma severity index scores (MASI) were made at
baseline and at 1, 2, 6, 12 and 24 months. The satisfaction index
(SI) and overall efficacy was also calculated.
Results: All 22 patients completed controls. The overall efficacy
was of 90% at all assessments, with no necessity of increasing
cream regimen of repeating laser resurfacing. MASI scores were
clear statistically and favorably significant (P < 0.001).
Conclusion: Pulsed fractional CO2 laser and topical cream
regimen obtained evident well-maintained results when combined
for melasma treatment, and it is most favorable in cases of dermal
location of pigment.
#263
HISTOPATHOLOGY AND
IMMUNOHISTOCHEMISTRY OF CUTANEOUS
LUPUS ERYTHEMATOSUS AFTER PULSED DYE
LASER TREATMENT
Teresa Truchuelo, Pablo Boixeda, Carmen Moreno,
María Luisa Zamorano, Javier Alcántara,
Pedro Jaén
Ramón y Cajal Hospital, Madrid, Spain
Background: Cutaneous lupus erythematosus (CLE) is an
autoimmune heterogeneous disorder with a wide range of skin
manifestations. Current treatment options include topical and
systemic approaches. Physical and surgical therapies including
cryotherapy, dermabrasion, and laser treatment have also been
tried. Few controlled prospective studies have been performed
with pulsed dye laser (PDL). Based on previous experience of our
group which supported the efficacy of PDL treatment in CLE, we
decided to study the histological changes induced by PDL. To
determine the efficacy of PDL in cutaneous lupus erythematosus
(CLE) lesions and the histological and immunohistochemical
changes.
Study: A prospective study was performed on nine patients with
histologically confirmed CLE (six chronic discoid CLE, two
tumidus CLE and one subacute CLE) who were treated with PDL
(595 nm, fluence 11 J/cm 2 , spot-size 7 mm, single pulse of
2 milliseconds). Biopsies were taken before, immediately after
and 4 weeks after treatment and they were stained with
hematoxylin–eosin and with commercially available antibodies
to the following endothelial cell adhesion molecules (ECAM):