Presidential Greeting - American Society for Laser Medicine and ...

46 American Society for Laser Medicine and Surgery Abstracts
groups underwent 8 weeks of CMS. Starting at week 6 and for the
remainder of the study, the Drug group was given daily injections
of Fluoxetine (10 mg/kg), and the TLT group was irradiated three
times a week (810 nm, 3 mm diameter, 350 mW peak power,
100 Hz with 20% duty circle, 2 min treatment). After 3 weeks of
treatment, a forced swimming test (FST) was performed. Rats
were weighed weekly. Animals were euthanized after 8 weeks.
Results: Starting at week 5 the no-stress group had significantly
higher body weight than all Stress groups (P < 0.05), there was no
difference in weight between the three Stress groups. However,
the Drug group had significantly less body weight than both
control and TLT groups (P < 0.05) after week 7. FST showed that
the Control group had significantly more immobility than the nostress
group (P < 0.05). Both Drug and TLT groups had
significantly less immobility than Control (P < 0.05). There was
no significant difference in immobility between the Drug and TLT
groups (P ¼ 0.62).
Conclusion: TLT was comparable with fluoxetine in improving
behavioral outcome after CMS. TLT did not cause weight loss,
which is consistently seen in patients treated with fluoxetine. This
study indicates that TLT may be an effective treatment for
depression (This study was funded by USU Intramural Concept
Award, RO704A).
#151
980 NM LASER IRRADIATION IMPROVED
FUNCTIONAL RECOVERY AFTER PERONEAL
NERVE INJURY IN RABBITS
Helina Moges, Xingjia Wu, Brian Pryor,
Jason Smith, Juanita Anders
Uniformed Services University of the Health Sciences, Bethesda,
MD; LiteCure, Newark, DE
Background: Light therapy (LT) has been shown to promote
peripheral nerve regeneration using wavelengths primarily
within 600–900 nm. Longer wavelengths are not commonly used
for repair of the peripheral nervous system and their effects in
promoting peripheral nerve regeneration have not been
adequately researched. Our objective in this study was to
investigate for the first time the capacity of 980 nm wavelength
laser irradiation to improve post-traumatic peripheral nerve
regeneration.
Study: Twelve White New Zealand rabbits were randomized into
two groups: (1) control, no light; and (2) LT, light treatment using
parameters: 980 nm wavelength, 1.5 W output power, 40 seconds
duration, and 8 cm 2 area at the skin surface. The left peroneal
nerve was completely transected and repaired using epineural
suture with 8-0 ethilon. Light was applied transcutaneously
immediately after closure of the skin and daily thereafter for a
total of 10 days. Control group was treated exactly the same but
with laser off. To measure functional recovery, toe-spreading
reflex test was performed at baseline and weekly starting from the
forth week post-injury. Animals were euthanized at week 9 postinjury.
Results: At week 4, toe spread was 63.7 2.6% and 65.3 2.9%
of baseline for control and LT groups, respectively, indicating
functional loss in both groups. At week 6, LT group showed
statistically significant functional recovery compared to week 4
(P < 0.05), while control group did not show functional recovery
(P ¼ 0.68). LT group consistently performed significantly better
in the toe spread reflex test compared to the control group starting
from week 6, with a return of function to 86.9 4.2% in LT group
and 72.2 3.6% in control group (P < 0.05) by week 9.
Conclusion: Our results demonstrate that 980 nm laser
irradiation successfully promoted earlier and faster functional
recovery after peroneal nerve transection and surgical repair.
This study warrants further investigations into use of 980 nm
lasers as treatment for nervous system injuries. (This research
was funded by the Comprehensive National Neuroscience
Program, Grant # G1704T.)
#152
COMBINED OCT AND MULTI-PHOTON
LUMINESCENCE MICROSCOPY FOR
MACROPHAGE DETECTION IN
ATHEROSCLEROTIC PLAQUES USING
PLASMONIC GOLD NANOROSE
Tianyi Wang, S.M. Shams Kazmi, Jordan Dwelle,
Veronika Sapozhnikova, Jake Mancuso,
Brian Willsey, Keith Johnston, Marc Feldman,
Andrew Dunn, Thomas Milner
University of Texas at Austin, Austin, TX; University of Texas
Health Science Center at San Antonio, San Antonio, TX
Background: Macrophages are an important cell type involved
in the progression of atherosclerosis which can lead to myocardial
infarction. We report combined OCT and multi-photon
luminescence (MPL) microscopy to visualize macrophages in
atherosclerotic plaques using plasmonic gold nanorose as a
biocompatible contrast agent.
Study: Atherosclerotic lesions were created in the aorta of a New
Zealand white rabbit subjected to a high cholesterol diet and
double balloon injury. The rabbit was injected with a suspension
of 30 nm diameter gold nanoroses coated with dextran. The
macrophages endocytosed nanoroses and ex vivo atherosclerotic
tissues were imaged by OCT and a custom-built scanning multiphoton
microscope. A mode-locked femtosecond Ti:Sapphire laser
(800 nm) was used as an excitation light source in the multiphoton
microscope, a band-pass filter (670–730 nm) and a
photomultiplier tube (PMT) were utilized to collect luminescence
emission.
Results: MPL images show the presence and location of
nanoroses, and are merged into co-registered OCT images to show
both plaque structure and composition.
Conclusion: Results of our study suggest that the spatial
distribution of macrophages endocytosing gold nanoroses in
atherosclerotic plaques can be identified by combined OCT and
MPL microscopy.
#155
THE EFFECTS OF RED LIGHT ON HIGH GLUCOSE
INDUCED DYSFUNCTIONAL MYOBLASTS AND
THEIR MECHANISM
Fang-Hui Li, Timon Cheng-Yi Liu, En-Xiu Wei,
Yan-Ying Liu
Laboratory of Laser Sports Medicine, South China Normal
University, Guangzhou, China
Background: Photobiomodulation promotes the wound healing
of diabetes. Summer sunlight improves diabetes. These
phenomena were simulated with the modulation of red light
(640 15 nm) at 0.35 W/m 2 from light emitting diode array for
15 min (RLED) on high glucose at 90 mmol/L induced
dysfunctional C2C12 myoblasts (DCMs) in this paper.