Presidential Greeting - American Society for Laser Medicine and ...

Study: The effects of RLED and resveratrol at 10 mmol/L (RES) on
the cell numbers, ATP activity, cell cycle, reactive oxygen species
level (ROS), mitochondrial membrane potential (MMP), the
mRNA expression of sirtuin 1 and manganese superoxide
dismutase (MnSOD) of DCMs were assessed in comparison with
C2C12 myoblasts in glucose at 22.5 mmol/L (CON).
Results: RLED promoted the mRNA expression of sirtuin 1
(P < 0.05) and MnSOD (P < 0.05). RLED, RES and N-acetyl
cysteine at 5 mmol/L (NAC) significantly reduced DCM ROS
(P < 0.05) at 18 h, increased DCM ATP activity (P < 0.05) at 72 h
and increased DCM MMP (P < 0.05) at 48 and 72 h, respectively,
among which the effects of RLED and RES were inhibited
(P < 0.05) by phosphatidylinositol 3-kinase (PI3K) inhibitor,
LY294002, respectively, and there were no significant effect
differences between RLED and RES.
Conclusion: Being similar with RES, the improvement of RLED
on DCMs might be mediated by sirtuin 1, MnSOD and PI3K.
#156
SIRTUIN1-MEDIATED PHOTOPROMOTION ON
TNF-ALPHA INHIBITED EXPRESSION OF
CIRCADIAN CLOCK GENES IN CULTURED
NIH3T3 FIBROBLASTS
Timon Cheng-Yi Liu, De-Feng Wu, Ling Zhu
Laboratory of Laser Sports Medicine, South China Normal
University, Guangzhou, China
Background: Tumor necrosis factor-alpha (TNF-alpha)
inhibited expression of the circadian clock genes has been found to
be modulated with low intensity 810 nm laser irradiation (LIDL)
in our laboratory. Its mechanism was further studied in this
paper.
Study: The expression of the circadian clock genes in NIH3T3
fibroblasts was synchronized by 50% horse serum shock for 2 h,
inhibited with TNF-alpha at 10 ng/ml, and then irradiated with
LIDL at 10 mW/cm 2 for 20 min. The mRNA expression of the
circadian clock genes and Sirtuin 1 (SIRT1), and the ratio of
nicotinamide adenine dinucleotide (NAD þ ) and its reduced form
NADH, NAD þ /NADH, were assessed every 6 h for 36 h.
Results: TNF-alpha inhibited the mRNA expression of Dbp
(P < 0.005) and Bmall (P < 0.05) at 18th hour, Per2 (P < 0.05) at
18th and 30th hours, Clock (P < 0.05) at 12th and 18th hours, and
SIRT1 (P < 0.05) at 18th and 24th hours, respectively, and
decreased NAD þ /NADH (P < 0.005) at 6th, 12th, 18th and 30th
hours, respectively. LIDL inhibited the effects of TNF-alpha on
Dbp (P < 0.05) and Per2 (P < 0.005) at 30th hours, Clock
(P < 0.05), Bmall (P < 0.05) and SIRT1 (P < 0.005) at 18th
hours, and NAD þ /NADH at 6th (P < 0.005), 12th (P < 0.05) and
18th (P < 0.05), respectively.
Conclusion: The promotion of LIDL on TNF-alpha inhibited
expression of the circadian clock genes may be mediated by
NAD þ /SIRT1. *It was supported by NSFC.
#158
American Society for Laser Medicine and Surgery Abstracts 47
A NEW POSSIBILITY TO TREAT SEVERE
TRACHEAL STENOSIS APPLYING LOW LEVEL
LASER THERAPY: PILOT STUDY
Nathali Pinto, Tatiana Magacho, Mara Pereira,
M. Cristina Chavantes
University of São Paulo, São Paulo, Brazil; Pitagoras University,
Vila Velha, Brazil
Background: Tracheal stenosis (TS) is a serious complication
that arises after endotracheal intubation’s problem,
tracheostomy, gastroesofagic reflux, etc. The most common benign
cause of upper airway stenosis, in all age groups, is postintubation
tracheal injury, which occurs in less than 5% of patients
submitted to long-term ventilation. Serious cases of severe
stenosis are very tough condition to be treated. Although,
untreated airway obstruction causes significant pulmonary
morbidity and it can progress to life-threatening airway
compromise. Several tracheal’s dilatations and surgery
(tracheoplasty) is a conventional treatment for such cases. In this
work, we aimed to investigate the Low Level Laser Therapy
(LLLT) effect in severe tracheal stenosis process, when all
previous treatment failure.
Study: Five cases w/resilient TS that had more than 75% of
tracheal obstruction, due to exaggerated granulation and scar
tissue, had received a previous traditional treatment without
success. They had complicated health conditions which forbidden
to undergoing to a new conventional surgical procedure. At first,
surgical laser was applied and afterwards LLLT w/? ¼ 685 nm,
P ¼ 35 mW, Dose ¼ 8 J/cm 2 were applied into endotracheal mode.
The time ranged according to tracheal lesion extent.
Results: The results showed that LLLT was extremely efficient to
control granulation tissue growth, even resilient scar tissue, due
to tracheal stenosis, was prevented through a bioinhibition effect
of LLLT. All patients were kept clear of tracheal stenosis, after 2
years, using lasers procedures. The histopathological specimens of
TS’ experimental study in vivo showed that LLLT was able to
control the tracheal mucosa healing process, preventing
inflammatory granulation tissue formation.
Conclusion: Laser accomplished to restore normal tracheal
lumen, due its biomodulation effect, as a less invasive treatment.
Thus, endoscopic laser management of tracheal stenosis, applying
LLLT, signalize be an effective method to control patient
predicament, successfully.
#159
LASER THERAPY FOR THE TREATMENT OF
CERVICAL PATHOLOGIES: A CLINICAL STUDY
Renato Jr. Liboro, Fred Kahn, Renato Liboro,
Fernanda Saraga
Meditech International, Inc., Toronto, Canada
Background: Cervical spine problems affect over 10% of the
adult population. They are generally characterized by pain which
impacts activity levels resulting in loss of time from work and
negatively affecting quality of life. Conditions encountered
include soft tissue and repetitive stress injuries, traumatic
episodes including whiplash injuries and herniated discs. The
wear and tear factors accompanying aging comprise a significant
percentage of the problems that present. If acute conditions are
not resolved in a timely manner, they become chronic,
compromising resolution with conventional therapies, including
pharmaceuticals and surgery. Recent, published literature
indicates that laser therapy has become established as an effective
therapeutic approach in the treatment of these problems,
resulting in the elimination of pain along with the restoration of
the quality of life and normal activity levels. In this study, we
examine the efficacy of laser therapy utilizing red and infrared
LED arrays along with laser probes as the choice of therapy.
Study: In a follow-up clinical study subsequent to our previously
published 2006 SPIE conference proceedings, we have analyzed a
cross-section of patients treated for a variety of neck problems