Presidential Greeting - American Society for Laser Medicine and ...

50 American Society for Laser Medicine and Surgery Abstracts
#167
NOVEL NON-INVASIVE TECHNIQUE USING LOW
LEVEL LASER FOR CHIN REJUVENATION
Vinod Podichetty, Jean-Claude Nerette
Research Practice Parterns, Inc., Miramar, FL; Bellissimo
Medical Center, Weston, FL
Background: Removal of excess fat pocket in the chin can
significantly define a lower facial structure but is often neglected
in rejuvenation efforts of the face and neck. A complete
rejuvenation of the neck should address contours in the chin area
reducing the subcutaneous fat to provide angularity between the
various planes of the lower face and neck. The aim of the study is
to evaluate the efficacy of low level laser therapy (LLLT) applied
on subcutaneous fat in the chin area in patients with undesirable
accumulation of submental fat.
Study: A total of 10 subjects were examined for the study. All
patients received LLLT using a AlGaInP laser diode source
(Meridian Medical, Inc., Vancouver, BC) at 658 nm wavelength
with a maximum output power of 30 mW/beam. Subjects received
five treatment sessions lasting 20 minutes each over a 2-week
period with a minimum follow-up of 6 months and post-treatment
assessment. Two clinicians performed the therapy, physical
examination, skinfold-caliper measurement and blinded
photographic evaluation.
Results: Laxity of the skin improved in all 10 patients.
Photographic assessment in 9 out of 10 patients studied revealed
significant changes in submental profiles after an average of four
treatment sessions. Mean degree of improvement (0 ¼ none,
1 ¼ mild, 2 ¼ moderate, 3 ¼ significant) was 2.8 ( 0.02).
Physician/subject assessment of fat loss (FL), skin tightening (ST),
chin profile (CP), and overall performance (OP) averaged a score of
9.5/9.0 (FL); 9.0/9.0 (ST); 8.5/9.0 (CP) and 9.5/9.5 (OP) (on a 1–10
scale; with 1 ¼ minimum; 10 ¼ maximum improvement). Results
were consistent at 6-month follow-up. One patient had transient
erythema and local swelling which resolved in 24 hours without
intervention.
Conclusion: Treatment choice to address skin changes and
subcutaneous fat deposition in chin area is typically surgical and
non-invasive options are currently unavailable. The current novel
study clearly demonstrates efficacy of low level laser in reducing
chin fat with restoration of neck contour and overall aesthetic
result.
PHOTODYNAMIC THERAPY
#168
IN VITRO STUDY OF THE PHOTODYNAMIC
THERAPY OF NANO-PHOTOSENSITIZER ON
HUMAN ORAL TONGUE CANCER CAL-27 CELLS
Guoyu Zhou, Pingping Li, Guolin Li, Yan Pang,
Lingyue Sheb, Qing Xu, Jizhong Gu, Xinyuan Zhu,
Michael R. Hamblin
College of Stomatology, Ninth People’s Hospital, Shanghai Jiao
Tong University School of Medicine; Shanghai Research Institute
of Stomatology; Shanghai Key Laboratory of Stomatology,
Shanghai, China; The First Affiliated Hospital of Haerbin
Medical University, Haerbin, China; College of Chemistry and
Chemical Technology, Shanghai Jiao Tong University, Shanghai,
China; Wellman Center for Photomedicine, Massachusetts
General Hospital, Boston, MA
Background: To study the effect of photodynamic therapy of
HPEE-ce6 nanoparticles on the human oral tongue cancer CAL-27
cells.
Study: Hyperbranched poly(ether-ester)-chlorin e6 nanophotosensitizer
(HPEE-ce6) were prepared by the reaction of
HPEE and ce6, and analyzed by confocal laser scanning
microscopy (CLSM) for cell internalization. With free chlorin e6
(ce6) for the control group, the phototoxicity of HPEE-ce6
nanoparticles on human oral tongue cancer CAL-27 cells was
detected by MTT assay.
Results: The nanoparticles with a diameter of ca. 50 nm were
synthesized by the covalent binding between hyperbranched
poly(ether-ester) (HPEE) and ce6. HPEE-ce6 were uptaken by
CAL-27 cells in vitro, mainly in cytoplasm. MTT assay showed the
significant effect of phototoxicity after comparison with ce6
control experiments (P < 0.05).
Conclusion: The results indicate that HPEE-ce6 could be used
as promising drug carriers in photodynamic therapy on CAL-27
cells.
#169
PDT DISINFECTION OF ORAL BIOFILM
Thomas Mang, Lynn Mikulski, Dharam Tayal,
Robert Baier
University at Buffalo, Buffalo, NY
Background: The ability of Photofrin mediated photodynamic
therapy to treat the bacterial strain S. mutans and different species
of Candida including Fluconazole and Amphotericin B resistant
isolates in planktonic cultures and on biofilm was studied.
Study: S. mutans were treated with PDT (630 nm) at 1.5, 3, 4.5, 9,
and 18.5 J/cm 2 after being incubated with Photofrin. An XTT
assay was performed on Biofilm grown on PMMA squares treated
with Photofrin and laser light (630 nm) at 9, 27, 54, and 63 J/cm 2 .
ATCC Candida strains C. albicans, C. glabrata, C. parapsilosis, C.
krusei, and C. tropicalis were examined along with antifungal
resistant isolates C. albicans, C. glabrata, C. guilliermondi, C.
tropicalis and C. krusei, for PDT susceptibility testing. The
resistant strains were from isolates from the oral cavities of
patients from a study on adults with AIDS. Candida (C. albicans,
C. glabrata and C. krusei) grown on SDA plates were inoculated
into appropriate media for planktonic experiments. Candida for
biofilm experiments were introduced to pre-conditioned PMMA
strips. Experiments were also performed using a flow cell system
and various substrata treated with radio frequency glow discharge.
Results: Experiments demonstrate that S. mutans have a high
level of susceptibility to the effects of Photofrin and 630 nm light
resulting in a statistically significant reduction in viability
(P < 0.0004; Mann–Whitney test). Light doses as low as 4.5 J/cm 2
resulted in a reduction of colonies to less than 0.1% of controls in
both planktonic and biofilm experiments. In Candida planktonic
and biofilm preparations PDT demonstrated significant killing in
both antifungal resistant strains. Experimental conditions
resulted in significant reductions in all strains (P ¼ 0.0006).
Conclusion: The results of this study, thus far, demonstrate the
efficacy of PDT for the treatment of oral microbes in planktonic or
biofilm conditions, including antifungal resistant isolate strains of
Candida.