Presidential Greeting - American Society for Laser Medicine and ...

after administration of targeted GNR. Additionally, the NIRNBI
images collected from targeted GNR tumors (n ¼ 4) showed a
220% increase in contrast compared to untargeted tumors.
Conclusion: We have demonstrated that a topical application of
gold nanorods targeted specifically to tumor growth factors results
in a significantly higher image contrast compared to non-targeted
gold nanorods. These results demonstrate the initial feasibility of
NIRNBI in demarcating tumor margins during surgical resection
using topical administration of targeted GNR.
#6
INDUCTION OF LIPOLYSIS IN HUMAN
ADIPOCYTES BY HYPERTHERMIA ALONE AND
IN COMBINATION WITH PHOTODYNAMIC
TREATMENT
Valery Tuchin, Irina Yanina, Georgy Simonenko,
Andrey Belikov, David Tabatadze, Ilya Yaroslavsky,
Gregory Altshuler
Institute of Optics and Biophotonics, Saratov State University
Saratov, Russian Federation; Palomar Medical Technologies, Inc.,
Burlington; Laser Center, SPITMO Saint-Petersburg, Russia
Background: Reduction of fat deposits is a goal of many
treatment approaches. Liposuction remains de facto goldstandard
procedure, providing high reduction in the volume of
adipose tissue in a short period of time. Minimally and noninvasive
alternatives to liposuction are highly desirable and are
being actively pursued by many groups world-wide. Objective of
this study was to investigate feasibility of inducing lipolysis in
human adipose tissue with hyperthermia alone and by combining
it with photodynamic treatment.
Study: Samples of human adipose tissue were subjected to
hyperthermia (up to 43.58C) using several temporal regimens of
heating and cooling down. In addition, some samples were stained
by photosensitizer Brilliant Green (maxima of absorption at 440
and 650 nm) and then irradiated by a two-color diode lamp
(W ¼ 70 mW/cm 2 at ? ¼ 442 nm and W ¼ 121 mW/cm 2 at
? ¼ 597 nm). The viability and status of the adipocytes were
assessed by light microscopy at several time points.
Results: Pronounced changes in shape and size of adipocytes
were registered. Observed dynamics of cell morphology is
consistent with hypothesis of induced lipolysis. Photodynamic
treatment accelerated the change significantly.
Conclusion: Hyperthermic treatment of adipocytes can be used
to induce lipolysis. The process can be accelerated by
photodynamic treatment. This technique has a potential for
minimally or non-invasive fat reduction in vivo.
#7
American Society for Laser Medicine and Surgery Abstracts 3
HEAT SHOCK PROTEIN EXPRESSION IN TISSUES
AFTER SHORT PULSE LASER-INDUCED DAMAGE
Amir Sajjadi, Kunal Mitra, Michael Grace
Melbourne, FL
Background: Effective laser-based therapeutics requires
detailed understanding of the biochemical mechanisms of laser–
tissue interaction. Therefore, we characterized the extents of
thermal damage and heat-affected zones following skin ablation
by analyzing the spatiotemporal distribution of heat shock
proteins.
Study: A focused-beam short-pulse laser was used to ablate the
skin surface of live anesthetized mice with minimal thermal
damage to surrounding untreated tissues. After ablation, the
extent of thermal ablation and the heat-affected zone was
analyzed by immunofluorescence localization of heat shock
proteins, the expression of which increases when cells are
exposed to elevated temperature or other stresses. Thermal
imaging was performed during laser irradiation to monitor
temperature effects in real time. To investigate the extent of
thermal damage and the initial events in healing, 47- and 70-kDa
heat shock proteins (HSP47 and HSP70) were localized in a
double-labeling procedure using HSP47- and HSP70-specific
primary antisera followed by Alexa-dye-labeled secondary
antisera. HSP analyses were performed at 0, 24, 36 and 48 hours
after laser irradiation. Images were obtained by laser scanning
confocal microscopy.
Results: Expression patterns of HSP70 and HSP47 delineated
the extent of thermal damage, and may illustrate the biochemical
process of wound healing. Analysis of the effects of laser
irradiation over the course of time using different laser
parameters followed by immunohistochemistry defines the time
course of laser effects on skin tissues. Extent of damage was
correlated with temperature rise using measured temperature
distribution and ablation depth for different laser parameters.
Conclusion: HSP70 and HSP47 can be simultaneously
visualized using immunofluorescence and laser scanning confocal
microscopy, and temporal changes in HSP expression patterns
may define both the laser-induced thermal damage zone and the
process of healing. Careful temporal analysis of HSP distribution
following laser irradiation supports the development of effective
new laser therapy protocols.
#8
MOBILIZATION OF ENDOTHELIAL PROGENITOR
CELLS FOLLOWING PHOTODYNAMIC THERAPY
Charles Gomer, Angela Ferrario, Marian Luna
University of Southern California, Los Angeles, CA;
Childrens Hospital Los Angeles, Los Angeles, CA
Background: Photodynamic therapy (PDT) mediated oxidative
stress induces direct tumor cell kill and, indirectly, modulates the
tumor microenvironment leading to local responses such as
inflammation, hypoxia and acute vascular injury. These local
reactions effect PDT tumor responsiveness by inducing
angiogenesis. To expand our understanding of PDT-mediated
tumor responses we are conducting a study to examine whether
PDT mobilizes bone marrow-derived vascular progenitor cells, a
mechanism associated with vasculogenesis, growth and
metastatic potential.
Study: We monitored the number of circulating endothelial cells
(CECs) and circulating endothelial progenitor cells (CEPs)
following PDT using four color flow cytometry. We also examined
the in vitro and in vivo expression of the chemokine stromal
derived factor-1 alpha (SDF-1a) and its receptor CXCR4, which
are key molecules associated with hematopoietic stem cell homing
and tumor angiogenesis.
Results: Flow cytometry results showed a twofold increase of
CECs and CEPs in the peripheral blood of Balb/c mice bearing 4T1
mammary tumors 24 hours after PDT when compared to
untreated controls. Western blot analysis of lysates from PDTtreated
cells showed, at 24 hours, a four- to fivefold increase in
expression of CXCR4 while no SDF-1a was detected in media by
ELISA. We observed increase of SDF-1a levels in plasma of
treated mice as well as activation of the SDF-1a receptor CXCR4
in 4T1 tumors soon after PDT.