Preface The expedition ARK XIX/3 with the German icebreaking RV ...

Species
Stations
Tmetonyx sp.1 326-1, 423
Tmetonyx sp.2 326-1
Tryphosella abyssalis 423
Tryphosella sp.3 412, 423, 448
Stegocephalidae
Gen. sp.1 448
Isopoda
Idoteidae
Saduria cf. sabini 412, 448
Molecular phylogeny and phylogeography of selected deep sea amphipod taxa
Specimens devoted to molecular analyses were put in absolute ethanol the soonest as
possible after sampling, preferably live when possible, in order to avoid possible DNA
degradation by enzymatic activity. As a rule, a very little part of each specimen selected
for molecular analyses was taken (usually the pereopod 6, as a whole or a part of it,
depending of the size of the animal). The amputated animals were preserved in absolute
ethanol as voucher specimens and for future morphological studies. DNA extractions
and purifications were carried out by means of QIAamp DNA Mini Kit (Qiagen), from
selected specimens of a total of at least 3 families, 7 genera and possibly 10 different
species, of which one could be a species new for science. In addition, DNA was
extracted from the isopod Saduria cf. sabini, abundantly caught in some baited traps.
The biological material consisted mostly of representatives of the superfamily
Lysianassoidea, of which at least 5 genera (Eurythenes, Boeckosimus, Paracallisoma,
Tmetonyx, and Tryphosella) and 8 species were obtained during ARK XIX/3c cruise.
The remaining material consisted of a few specimens of related amphipod families
(Stegocephalidae, Eusiridae), and was processed for the sake of outgroup use in future
molecular phylogenetic reconstructions. All this material will be processed in the
laboratory, in order to obtain DNA fragment sequences of at least 18S, COI and possibly
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