Experimental Study of Biodegradation of Ethanol and Toluene Vapors

1
φ
e
= ( −φ
x
) + meC
x
(5-17)
Y
max
ex
where the relations for the defined maximal yields and maintenance, and the metabolic
model parameters (K,δ, m ATP ) are the same forms as indicated in Equations (5-15a) and
(5-15b).
5.2 Model for Benzyl Alcohol Bioremediation
5.2.1 Metabolic Equations
The major metabolic activities taking place in benzyl alcohol bioremediation are
shown in Figure 5-2 (developed in this work) and are described below.
(1) Biomass Synthesis from Benzyl Alcohol
It is assumed that in the synthesis of biomass, 0.27 mol CO 2 is produced per C-
mole biomass synthesized (Gornmers et al., 1988). The stoichiometry of the biomass
formation reaction from benzyl alcohol is as follows (expressed as 1 C-mol of benzyl
alcohol and 1 C-mol of biomass):
−1.27CH 8 / 7O1/
7
− 0.2NH
3
− α1B ATP − 0.758H
2O
−1.
084NAD
+
CH
7 / 5O2 / 5N1/
5
+ 1.084NADH
2
+ 0. 27CO2
+ α1B
ADP = 0 (5-18)
where
mATP
α1 B
= K + (when μ>0 ) is assumed to be the same form as described in
μ
Equation (5-4).
(2) Catabolism of Benzyl Alcohol
For benzyl alcohol, based on its pathway (See Appendix A-III), the catabolism
is represented by the following reactions:
C 7 H 8 O + 2NAD +3H 2 O + 2O 2
Benzyl alcohol
C 3 H 4 O 3 + C 2 H 4 O + CH 2 O 2 + CO 2 + 2NADH 2 (5-19)
Pyruvate Acetaldehyde Formate
88