Experimental Study of Biodegradation of Ethanol and Toluene Vapors
Experimental Study of Biodegradation of Ethanol and Toluene Vapors
Experimental Study of Biodegradation of Ethanol and Toluene Vapors
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
<strong>Ethanol</strong>, biomass <strong>and</strong><br />
ammonium concentrations,<br />
g/L; O 2 consumption <strong>and</strong><br />
CO 2 formation, g/L-h<br />
7<br />
6<br />
5<br />
4<br />
3<br />
2<br />
1<br />
0<br />
S<br />
C n<br />
r ATP<br />
X<br />
Qc Qo<br />
0 10 20 30<br />
Time, h<br />
0.1<br />
0.08<br />
0.06<br />
0.04<br />
0.02<br />
0<br />
ATP consumption, mol/L-h<br />
Figure 5-11(c). Prediction <strong>of</strong> batch growth on ethanol at initial concentration <strong>of</strong> 6 g/L<br />
5.5.2 Batch Growth on Benzyl Alcohol<br />
The specific growth rate <strong>of</strong> Pseudomonas putida on benzyl alcohol was assumed<br />
to follow the following substrate inhibition equation (4-10):<br />
b,max<br />
b<br />
b n<br />
μ −<br />
(4-10)<br />
= μ<br />
K<br />
b<br />
C<br />
+ C<br />
b<br />
(1<br />
C<br />
C<br />
b,max<br />
)<br />
The values <strong>of</strong> parameters in Table 5-2 are applied in Equation (4-10). Figure 5-12<br />
shows the effect <strong>of</strong> benzyl alcohol concentration on the specific growth rate. The results<br />
demonstrate that at low concentration, the specific growth rate increases with increase<br />
<strong>of</strong> benzyl alcohol concentrations. However, after the concentration <strong>of</strong> benzyl alcohol<br />
reaches 1.2 g/L, the specific growth rate starts decreasing with further increase <strong>of</strong><br />
benzyl alcohol concentration. When the benzyl alcohol concentration reaches C b,max (2.9<br />
g/L), the cells cease to grow, <strong>and</strong> the specific growth rate equals zero.<br />
125