Experimental Study of Biodegradation of Ethanol and Toluene Vapors
Experimental Study of Biodegradation of Ethanol and Toluene Vapors
Experimental Study of Biodegradation of Ethanol and Toluene Vapors
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2.4.1 Batch Reactor<br />
In a batch reactor, the reactants are initially added into the reactor <strong>and</strong> left to<br />
react for a certain period <strong>of</strong> time. The reactant can be either a pure chemical reaction or<br />
a biochemical one. The content <strong>of</strong> the reactor is kept mixed in order to have a uniform<br />
composition throughout the reactor. The process is unsteady state (changes with time)<br />
<strong>and</strong> the reactor content is discharged at the end <strong>of</strong> the process. The performance<br />
equation <strong>of</strong> a batch reactor can be derived from the material balance <strong>of</strong> substrate as<br />
(Levenspiel, 1972):<br />
dX = r (2-13)<br />
x<br />
dt<br />
dS<br />
dt<br />
r s<br />
= −<br />
(2-14)<br />
in which t is the time, X is the biomass mass concentrations <strong>and</strong> r x is the rate <strong>of</strong> biomass<br />
formation, S is the substrate mass concentrations <strong>and</strong> r s is the rate <strong>of</strong> substrate<br />
consumption. Solving Equations (2-13) <strong>and</strong> (2-14) will give the concentrations <strong>of</strong><br />
biomass (X) <strong>and</strong> substrate (S) at any desired time. If the temperature <strong>of</strong> the reactor<br />
changes, an energy balance will also be necessary for solving the performance equation<br />
<strong>of</strong> the batch reactor.<br />
2.4.2 Fedbatch Reactors<br />
In a fedbatch (semi-batch) reactor, an initial amount <strong>of</strong> reactant <strong>and</strong>/or medium<br />
is charged into the reactor (initial charge) which is also kept well mixed. More reactants<br />
or medium are added to the reactor as the reaction proceeds. The process is unsteady<br />
state <strong>and</strong> the contents <strong>of</strong> the reactor need to be discharged after a certain period <strong>of</strong> time.<br />
Fedbatch processes are <strong>of</strong>ten used in fermentation to minimize substrate inhibition, or to<br />
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