Experimental Study of Biodegradation of Ethanol and Toluene Vapors

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Heinen, A. W.; J. A. Peters, and H. van Bekkum, 2000. “Competitive adsorption of water and toluene on modified activated carbon supports”, Appl. Catalysis A: General, 194-195:193-202. Hekmat, D., and D. Vortmeyer, 1994. “Modelling of biodegradation processes in trickled-bed bioreactors”, Chem. Eng. Sci., 49(24A):4327-4345. Hill, G. A., 1974, Kinetics of Phenol Degradation by Pseudomonas putida. M.Sc Thesis, University of Waterloo. Hill, G.A. and C.W. Robinson, 1975. “Substrate Inhibition Kinetics: Phenol Degradation by Pseudomonas putida”, Biotechnol. Bioeng. 17:1599-1615. Hill, G.A., B. J. Ritchie, H. Baheri, and S. Textor, 1996, Mixed-Flow Hydrodynamics: Advances in Engineering Fluid Mechanics Series, Nicholas P. Cheremisinoff, Editor, Gulf Publishing Company, pp 499-519. Hill, G. A., and A. J. Daugulis, 1999. “Phenol inhibition kinetics for growth of Acetobacter aceti on ethanol”, Appl. Microbiol. Biotechnol, 51: 841-846. Hodge, D. S.; J. S. Devinny, 1994. “Biofilter treatment of ethanol vapors”, Environ. Prog., 13:167-173. Hounsell, G., 1995. “Case studies: selection of high efficiency VOC removal technologies for process air streams”, Proceedings, Annual Meeting - Air and Waste Management Association, Vol. 1A, page 95-MP3.01, 24 pp. Hussey, F., and A. Gupta, 1998. “Meet VOC regs with the right adsorbent”, Ind. Paint & Powder, 3: 6-50. Hunt, C.; P. Alvarez; R. dos Santos Ferreira, and H. Corseuil, 1997. “Effect of ethanol on aerobic BTEX degradation”, In: In situ and Onsite Bioremediation, B. C. Alleman and A. L. Leeson (Eds.). Batelle Press, Columbus, OH, Vol. 1, pp. 49-54. Jorio, H., K. Kiared, R. Brzezinski, A. Leroux, G. Viel , and M. Heitz, 1998. “Treatment of air polluted with high concentrations of toluene and xylene in a pilot-scale biofilter”, J. Chem. Technol. Biotechnol., 73:184 – 196. Kastner, J. R.; D. N. Thompson, and R. S. Cherry, 1999. “Water-soluble polymer for increasing the biodegradation of sparingly soluble vapors”, Enzyme Microbial. Technol., 24: 104-110. Kennedy, J. B. and Neville, A. M., 1974, Basic Statistical Methods for Engineers and Scientists, 2 nd Edition, Harper and Row Publishers. 160
Keuning, S., and D. Jager, 1994. “Simultaneous degradation of chlorobenzene, toluene, xylene, and ethanol by pure and mixed Pseudomonas cultures”, in Appl. Biotechnol. for Site Remediation, Hinchee, R.E., D.B. Anderson, F.B. Metting and G.D. Sayles (Eds.), Lewis Publishers, Boca Raton, USA, pp. 332-336. Kiared, K.; L. Bibeau; and R. Brzezinski, 1996. “Biological elimination of VOCs in biofilter”, Environ. Prog., 15 (3):148 – 152. Kim, H.-S. and P.R. Jaffe, “Simulating the biodegradation of toluene at the macroscopic and pore-level scale for aerobic and nitrate reducing conditions”, ACS National Meeting, Division of Environ. Chem. Preprints of Extended Abstract, 42(2): 363-365, August, 2000. Kirchner, K.; G. Hauk, and H. J. Rehm, 1987. “Exhaust gas purification using immobilized monocultures (biocatalysts)”, Appl. Microbiol. Biotechnol., 26:579-587. Kirchner, K.; U. Schlachter, and H. J. Rehm, 1989. “Biological purification of exhaust air using fixed bacterial monocultures”, Appl. Microbiol. Biotechnol., 31: 629-632. Kirchner, K.; C. A. Gossen, and H. J. Rehm, 1991. “Purification of exhaust air containing organic pollutants in a trickled-bed bioreactor”, Appl. Microbiol. Biotechnol., 35: 396-400. Kirchner, K.; S. Wagner, and H. J. Rehm, 1992. “Exhaust gas purification using biocatalysts (Fixed bacteria monocultures) – the influence of biofilm diffusion rate (O2) on the overall reaction rate”, Appl. Microbiol. Biotechnol., 37: 277-279. Kirchner, K.; S. Wagner, and H. J. Rehm, 1996. “Removal of pollutants from exhaust gases in the trickle-bed bioreactor. Effect of oxygen”, Appl. Microbiol. Biotechnol., 45: 415-419. Klainer, A. S. and C. J. Betsch, 1970. “Scanning –Beam Eletron Microscopy of Selected Microorganisms”, J. Infectious Disease, 121(3):339-343. Kozliak, E. I.; T. L. Ostlie-Dunn, 1997. “Efficient air purification from VOC using a fiber-based trickle-bed bioreactor”, Preprints Am. Chem. Soc., Division of Petroleum Chem., 42(3): 694-698. Kozliak, E. I.; T. L. Ostlie-Dunn, M. L. Jacobson, S. R. Mattson; and R. T. Domack, 2000. “Efficient steady-state volatile organic compound removal from air by live bacteria immobilized on fiber supports”, Bioremediation J., 4(1):81-96. 161
Page 1 and 2:
BIOREMEDIATION OF VOLATILE ORGANIC
Page 3 and 4:
ABSTRACT The mass transfer of ethan
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effect on the growth of P. putida.
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ACKNOWLEDGMENT Of the countless peo
Page 9 and 10:
TABLE OF CONTENTS PERMISSION TO USE
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4.3 Batch Growth on Ethanol and Ben
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LIST OF FIGURES Figure 3-1. Schemat
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Figure 5.3 (b) Simulation of ethano
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Figure 5-16. Continuous removal of
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NOMENCLATURE a - Interfacial area,
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CHAPTER ONE - INTRODUCTION 1.1 Air
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esults (van Groenestijn and Hesseli
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experimental results are presented
Page 27 and 28:
Table 2.2 VOC Emissions in the U.S.
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(paint shop effluents, for instance
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combustion provides 70 to 90% air p
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used to degrade ethanol vapours and
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g m -3 . This would suggest that th
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treatment, This excessive biomass f
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The simultaneous degradation of tol
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emoves only water-soluble gases. Wa
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2.2.6 Pseudomonas It is well known
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2.3.2 Growth Models with Inhibition
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where µ is the total specific grow
Page 49 and 50:
2.4.1 Batch Reactor In a batch reac
Page 51 and 52:
discharged from the other end. The
Page 53 and 54:
element solution consisted of (gram
Page 55 and 56:
growth on ethanol, 20-48 hours for
Page 57 and 58:
Gas phase samples of toluene were c
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Inlet sampler Exit sampler To fume
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Once the oxygen meter indicated a s
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centrifuged and the supernatant was
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where k L a is the volumetric mass
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Figure 4-2a shows ethanol liquid co
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coefficient (k L a) in the well-mix
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calculated by dividing the gas volu
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Average bubble diameter, mm 8 7 6 5
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Bioflow III bioreactor. Equation (4
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3.5 y=Ln(S0/S) 3 2.5 2 1.5 1 y = 12
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Ethanol and biomass concentration,
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4.3.2 Batch Growth on Benzyl Alcoho
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Biomass and benzyl alcohol concentr
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ethanol loading was beyond the maxi
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Table 4-2. Comparison of bioremedia
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Biomass, benzyl alcohol and toluene
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4.4.3 Toluene-ethanol Bioremediatio
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Substrate and biomass concentration
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Biomass and benzyl alcohol concentr
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Biomass and benzoic acid concentrat
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ethanol. These results clearly indi
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CHAPTER FIVE - MATHEMATICAL MODELIN
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The stoichiometry of the biomass sy
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synthesis and in the ethanol catabo
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1 max Y ex = − 0.635 + 2δ + K 3
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Biomass Eq.5-18 Benzyl Alcohol Eq.5
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r x r N -1.27 -1 0 1 0 0 -0.2 0 0 2
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φ = −θ b ⋅ν (5-31) φ b = 1.
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The data sets used to evaluate the
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Figures 5.4a and 5.4b present 95% c
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Ethanol and biomass concentration (
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Ethanol measured (C-mol/L) 0.3 0.25
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Figures 5.5 (a-d) demonstrate the s
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Benzyl alcohol and biomass concentr
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Benzyl alcohol measured (C-mol/L) 0
Page 129 and 130: x = 5 ∑ i= 1 x N i (5-40) where x
Page 131 and 132: ethanol in a steady state mode is l
Page 133 and 134: where ν 1 , ν 2 , ν 3, ν 4 are
Page 135 and 136: Biomass concentration, g/L 1.5 1 0.
Page 137 and 138: φ T =ν 4 (5-53b) φx = −ν 1 (5
Page 139 and 140: In this study, a metabolic model ha
Page 141 and 142: Equations (5-34) and (5-35) and the
Page 143 and 144: ATP 1 = Y max ATP r x + m ATP ⋅ C
Page 145 and 146: Ethanol, biomass and ammonium conce
Page 147 and 148: Figure 5-13a shows that the lower t
Page 149 and 150: Biomass, substrate concentrations,
Page 151 and 152: C e DKe = (5-63) μ − D m Equatio
Page 153 and 154: corresponds with the value associat
Page 155 and 156: Biomass and ethanol concentrations
Page 157 and 158: than 4.0 mg/L at all dilution rates
Page 159 and 160: Biomass, benzyl alcohol and ammonia
Page 161 and 162: negligible ( y out , e = 0) , and e
Page 163 and 164: Figure 5-17 shows the results at a
Page 165 and 166: Biomass Concentrations, g/L 3.5 3 2
Page 167 and 168: C x ( 1 max Q max = ⋅{ D( Cb0 −
Page 169 and 170: 1.2 g/L, the system could not be op
Page 171 and 172: The continuous models can capture a
Page 173 and 174: • Air stripping coefficients obta
Page 175 and 176: Toluene acts as an inhibitor on the
Page 177 and 178: Bruining, W. J., G. E. H. Joosten,
Page 179: Falatko, D. M., and J. T. Novak, 19
Page 183 and 184: Orshansky, F., and N. Narkis, 1997.
Page 185 and 186: Togna, A. P, M. Singh, 1994. “Bio
Page 187 and 188: APPENDIX A. Important Metabolic Pat
Page 189 and 190: III. Toluene Pathway (Adapted from
Page 191 and 192: Appendix B. Calibration Procedure a
Page 193 and 194: Figure B-2. Pseudomonas putida (ATC
Page 195 and 196: Appendix B-3. Calibration of Chemic
Page 197 and 198: Appendix B-4. Calibration of Toluen
Page 199 and 200: Appendix C. Experimental Results: A
Page 201 and 202: Probe response time, s 12 10 8 6 4
Page 203 and 204: Appendix C-2. Fed-batch Operation B
Page 205 and 206: Appendix D: Mathematical Solutions
Page 207 and 208: The microscopic model allows the fo
Page 209 and 210: 13δ Y o , = 0.585 2 x = (D-24) (8K
Page 211 and 212: 2 σ = −1.3E − 06 (E-11) Y xe Y
Page 213: Biomass, ethanol, and acetic acid c
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Experimental Study of Biodegradation of Ethanol and Toluene Vapors

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