Experimental Study of Biodegradation of Ethanol and Toluene Vapors

Coupling the metabolic Equations (5-19) – (5-23), the overall catabolism of benzyl
alcohol can then be represented by:
C 7 H 8 O + 13 NAD + 9 H 2 O + 2O 2 13 NADH 2 + 7 CO 2 (5-24)
Equation (5-24) can be rearranged based on 1 C-mol benzyl alcohol as:
9 2 13 13
− CH
8 / 7O1/
7
− H
2O
− O2
− NAD + NADH
2
+ CO2
= 0 (5-25)
7 7 7 7
(3) Oxidative Phosphorylation
The net amount of NADH 2 produced in the biomass precursor synthesis and in
the benzyl alcohol catabolism reaction is consumed to yield ATP according to equation
(5-11):
− NADH
1
− O
2
− δ ADP + H O + δATP
+ NAD 0
(5-11)
2 2
2
=
5.2.2 Mathematical Modeling
Similar to the procedure used in Section 5.1.2, the reaction Equations (5-18),
(5-25) and (5-11) can be written in condensed form using the stoichiometric matrix θ
b
,
where the first compound is taken to be benzyl alcohol, followed by biomass, ammonia,
oxygen, carbon dioxide, water, ATP, and NADH 2 .
The conversion rates can be expressed as a function of the reaction rates by a set
of linear equations with parameters composed of the stoichiometric coefficients (Roels,
1983). The stoichiometric matrix θ b
for three reactions (5-18), (5-25) and (5-11) can
be written with 1 C-mol basis as:
90