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Experimental Study of Biodegradation of Ethanol and Toluene Vapors

Experimental Study of Biodegradation of Ethanol and Toluene Vapors

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oxygen is supplied, the system cannot be operated at steady state under these<br />

conditions.<br />

4. Low dilution rates generate low biomass concentrations because ethanol is<br />

consumed for maintenance requirements.<br />

5.6.2 Theoretical Predictions <strong>of</strong> Steady State Continuous Removal <strong>of</strong> Benzyl Alcohol<br />

The relation between the specific growth rate <strong>and</strong> benzyl alcohol concentration<br />

was given by:<br />

b,max<br />

b<br />

b n<br />

μ (1 − )<br />

(4-3)<br />

= μ<br />

K<br />

b<br />

C<br />

+ C<br />

b<br />

C<br />

C<br />

b,max<br />

The biomass concentration can be predicted by combining<br />

− φ = μC<br />

<strong>and</strong><br />

x<br />

x<br />

φ = D( C<br />

0<br />

− Cb ) with Equation (5-32):<br />

b<br />

b<br />

C<br />

x<br />

D( Cb0<br />

− Cb<br />

)<br />

= (5-69)<br />

( μ / Y + m )<br />

xb<br />

b<br />

Using the values <strong>of</strong> parameters obtained for benzyl alcohol bioremediation listed<br />

in Table 5.2 with the operating conditions <strong>of</strong> the reactor used in this study<br />

( Q 24L<br />

/ h;<br />

V = R<br />

1.5L)<br />

, benzyl alcohol removal in a CSTR at steady state can be<br />

=<br />

predicted by Equations (4-3) <strong>and</strong> (5-69) as a function <strong>of</strong> dilution rate <strong>and</strong> feed rate.<br />

Results are shown in Figures 5-15a-c. Figure 5-15a reveals that the system can be<br />

maintained at steady state for dilution rates up to 0.25 h<br />

-1 . When the dilution rate is<br />

higher than 0.25 h -1 , the cells are washed out (X=0) <strong>and</strong> the substrate concentration<br />

reaches the same as the feed (C b = C b0 ). The results also indicate that DO level is higher<br />

136

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