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FAME (g/100g)<br />

FAME (g/100g)<br />

FEEDING OMEGA-6 AND OMEGA-3 FATTY ACIDS IN EARLY LACTATION<br />

focused on the effect <strong>of</strong> dietary 22:6n-3 on BP or FF composition but comparability to FO<br />

studies, at least for MF, has been suggested (AbuGhazaleh et al., 2009). In a study<br />

observing the FA composition in FF collected in the abattoir from crossbred heifers,<br />

higher 20:5n-3 <strong>and</strong> 22:6n-3 was found when supplementing FO at 2.08 <strong>and</strong> 41.5 g/kg<br />

DM <strong>of</strong> the diet (Childs et al., 2008). Zeron et al. (2002) found an enrichment <strong>of</strong> 18:2n-6<br />

<strong>and</strong> 22:6n-3 in the PL fraction <strong>of</strong> FF when feeding FO to ewes. Whereas CE <strong>and</strong> PL in<br />

HDL seem to be a very poor substrate for the mammary lipoprotein lipase, the HDL<br />

fraction is the largest lipoprotein class in BP (Bauchart, 1993) <strong>and</strong> FF (Brantmeier et al.,<br />

1987; Wehrman et al., 1991), therefore allowing substantial enrichment in 22:6n-3 as<br />

proven the current <strong>and</strong> aforementioned studies.<br />

0.25<br />

Cholesterol Esters<br />

0.6<br />

Non Esterified Fatty Acids<br />

0.20<br />

0.15<br />

0.10<br />

0.05<br />

0.00<br />

1.8<br />

1.6<br />

1.4<br />

1.2<br />

1.0<br />

0.8<br />

0.6<br />

0.4<br />

0.2<br />

0.0<br />

a<br />

b<br />

Phospholipids<br />

a<br />

a<br />

b<br />

b<br />

b<br />

b<br />

Triacylglycerols<br />

-7 14 46 -7 14 46<br />

DIM<br />

DIM<br />

Figure 6. The 22:6n-3 content in g/100g fatty acid methyl esters (FAME) <strong>of</strong> separated blood plasma lipid<br />

classes <strong>of</strong> cows fed palm prills (CON:■), sequential short chain n-6 <strong>and</strong> n-3 fatty acids (SHORT:▲) or<br />

sequential long chain n-6 <strong>and</strong> n-3 fatty acids (LONG:●) during the transition (n-6) <strong>and</strong> early breeding (n-3)<br />

period. Differences between diets within the same day are indicated by different labels.<br />

0.5<br />

0.4<br />

0.3<br />

0.2<br />

0.1<br />

0.0<br />

0.45<br />

0.40<br />

0.35<br />

0.30<br />

0.25<br />

0.20<br />

0.15<br />

0.10<br />

0.05<br />

0.00<br />

a<br />

b<br />

b<br />

a<br />

b<br />

185

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