Central Rice Research Institute Annual report...2011-12

Characterization and maintenance of
landraces, identified donors and pre-breeding
lines for upland ecosystem
Molecular characterization of 104 upland rice
germplasm comprising of traditional cultivars and released
varieties, was carried out using 43 SSR markers
which were polymorphic across the genotypes. A total
of 109 alleles were detected with an average of 2.53
alleles per locus and 10 were found to be rare alleles.
Out of 43 primers, only one marker (RM23) showed
four alleles, 21 primers showed three alleles and remaining
21 primers showed two alleles (Fig. 1). Dendrogram
constructed using UPGMA resulted in 7 clusters
at 1.5 linkage distances. Cluster I consisted of RR345-
2, Clsuter II consisted of Salumpikit, Cluster III consisted
of Abhishek, RR218-65 and Vanaprabha, Cluster
IV consisted of Sadabahar, RR158-24, RR348-2 and
NDR 1045-2, Cluster V consisted of DDR 105,
Birsadhan 101, CT 13370-12-2-M UPLRi-7 and VLDT-
1, Cluster VI consists of RR20-5 and Azucena,
Lalnakanda and Cluster VII consists of P 0013, IAC25,
B6144F-MR-6, Palawan and Tulasi. The same set of
genotypes was evaluated for 23 morphological characters
(Table 1).
Table 1. Distribution of genotypes into different classes for different characters studied
Leaf sheath colour Leaf blade colour Leaf pubescence Panicle exsertion
Green 71 Light green 8 Glabrous 15 Well exserted 61
Purple lines 2 Green 70 Intermediate 67 Moderately exserted 20
Light purple 24 Dark green 25 Pubescent 22 Just exserted 15
Purple 7 Purple margin 1 Partly exserted 8
Stigma colour Epiculus colour Panicle type Awning
White 73 White 57 Compact 97 Short and partly awned 96
Yellow 2 Straw 19 Intermediate 5 Short and fully awned 7
Light purple 17 Brown 2 Open 2 Long awned 1
Purple 12 Red 17
Red apex 1
Purple 5
Purple apex 3
RM340
RM205
RM153
Fig. 1. Molecular profiling of different genotypes using SSR primers RM340, RM205 and RM153
18 CRRI ANNUAL REPORT 2011-12