Central Rice Research Institute Annual report...2011-12

of Pi9 and Pi ta2, however, would provide broad spectrum
resistance to all isolates evaluated.
Morpho-physiological and Molecular
Characterization of Microflora and
Development of Database
Polymorphism in Fusarium isolates associated
with seeds of rice cultivars
Polymorphic bands were observed by RFLP of Inter-geneic
spacer (IGS) region of ribosomal DNA of
Fusarium isolates, associated with seeds of rice cultivars.
Fumonisins producing and non- fumonisins producers
clustered in different groups (Fig. 27).
RAPD primers ap12h, P-160, PU-1, P-117, R3, R-
108, R-1,R-2, PU-2, PU-3,P-54, OPB-10 and 3B were used
to amplify seed borne Fusarium isolated from various
rice cultivars. High degree of polymorphism was observed
among Fusarium isolates (Fig. 28).
Evaluation of toxins/other metabolites
produced by fungi on host genotypes
Endophytic Penicillium from salt tolerant rice cultivar
Lunisree protected rice seedlings against seedling
mortality caused by Fusarium spp. This isolate was also
effective against virulent isolates of ‘rice blast pathogen’
in vitro. First spray of cell free aqueous extract of
false smut balls on rice cultivar Ranjit at active tillering
stage and second spraying just before panicle
emergence were effective. About 10% increase
in yield was recorded.
Characterization and development of
database for bacterial blight
One hundred isolates of bacterial leaf blight
collected from different locations in Odisha viz.,
Bolangir, Sonepur, Kalahandi, Nawapara,
Keonjhar, Malkangiri, Nawarangpur,
Fig. 27. Restriction digestion and PAGE of IGS region of
seedborne Fusarium by EcoRI and HhaI.
Fig. 28. Polymorphism in Fusarium isolates amplified by 13 RAPD primers
80 CRRI ANNUAL REPORT 2011-12