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Central Rice Research Institute Annual report...2011-12

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Strategic <strong>Research</strong> on Pathogens/Pest Population Dynamics, Crop Losses and Forecasting<br />

Mayurbhanj, Phulbani, Boudh, Puri, Sambalpur,<br />

Bargarh, Deogarh and Sundergarh were morpho-physiologically<br />

characterized by testing their pathogenicity<br />

on the differential cultivars IR 8(Xa11), IR 20(Xa4), BJ<br />

1(xa13), DV 85(xa5+Xa7), Cemposelak (Xa3) and Java<br />

14 (Xa1+Xa3) under field conditions. Nine pathotypes<br />

viz., xa1 (42 isolates), xa2 (18 isolates), xa3 (14 isolates),<br />

xa5 (seven isolates), xb1 (four isolates), xb5 (five isolates),<br />

xb4 (three isolates), xb14 (two isolates), xf1 (five<br />

isolates) were identified based on their reaction on the<br />

differentials. Among the pathotypes, xa1 was the most<br />

virulent one which produced the susceptible reaction<br />

on all the differential cultivars and the pathotype xf1<br />

was the most avirulent type showing susceptible reaction<br />

only on one variety. The pathotypes xa2, xa3, xa5,<br />

xb1 and xb5 were the moderately virulent types producing<br />

susceptible reaction on 5 and 4 differential cultivars,<br />

respectively. The virulence study of the isolates<br />

revealed that there was wide variability in the population<br />

structure of Xanthomonas oryzae pv. oryzae and both<br />

the virulent and avirulent types coexisted in the population<br />

of the pathogen in a locality.<br />

Morpho-physiological, molecular characterization<br />

and development of database for the<br />

microflora<br />

The nucleotide sequences of different microflora isolates<br />

were submitted to NCBI-Gen Bank and accession<br />

numbers were obtained (Table 33).<br />

Table 33. NCBI Gen Bank accession numbers of microbes<br />

NCB I-GenBank<br />

accession number Organism Description<br />

Endophytes from rice seeds<br />

JQ039898 Dendryphiella Endophyte associated with seeds of rice landrace<br />

Bora Saruchina collected from Jajpur district<br />

of Odisha<br />

JQ039897 Unclassified Fungus Endophyte associated with seeds of rice landrace<br />

Rajmali collected from Jajpur district of Odisha<br />

JQ814883 Leptosphaeria Endophytic fungal cultures associated with rice<br />

landrace Sanrasi from Mayurbhanj district<br />

of Odisha<br />

JQ753708 Unclassified endophytic Endophytic fungus growth promoter for rice<br />

Fungus<br />

seedlings and -antagonistic to rice pathogens<br />

<strong>Rice</strong> pathogens<br />

JQ753703 Curvularia spp. Curvularia spp. causing leaf spots on Paspalum<br />

scrobiculatum and seedling mortality of rice<br />

JQ039899 Bipolaris sorokiniana Bipolaris sorokiniana causal organism of brown spots<br />

on scented rice cultivars in Odisha<br />

JQ753704 Bipolaris sorokiniana Seedling mortality in hybrid rice parental lines<br />

caused by Bipolaris sorokiniana<br />

JQ753705 Cochliobolus miyabeanus Brown leaf spot on rice var. Kalajeera<br />

JQ753706 Cochliobolus miyabeanus Brown leaf spot on rice var. Nua Dhusara<br />

JQ753707 Cochliobolus miyabeanus Seedling mortality in hybrid rice caused by<br />

Cochliobolus miyabeanus<br />

Biocontrol agents-antagonistic bacteria from cowshed<br />

JQ753710 Bacillus vallismortis Cowshed bacteria Bacillus vallismortis antagonistic<br />

to molds and Fusarium species<br />

JQ753711 Bacillus amyloliquefaciens Cowshed bacteria Bacillus amyloliquefaciens subsp.<br />

subsp. planta<br />

Planta antagonistic to molds and Fusarium species<br />

CRRI ANNUAL REPORT 2011-<strong>12</strong><br />

81

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