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Central Rice Research Institute Annual report...2011-12

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jected to different levels of sodium chloride solution<br />

(Fig. <strong>12</strong>), however, the C δ value at high salinity environment<br />

shows indication of C 4<br />

ness of S. fruticosa (Table<br />

19).<br />

One month old seedlings of the same germplasm<br />

accessions were exposed to vegetative stage moisture<br />

stress under field condition with similar checks as that<br />

of seedling stage stress. Soil moisture content varied<br />

from 2.6 to 9.7% under 30 cm soil depth with soil moisture<br />

tension of more than 60 kPa. Leaf death score was<br />

Control <strong>12</strong> ds m -1 18 ds m -1<br />

24 ds m -1 30 ds m -1 36 ds m -1<br />

Fig. <strong>12</strong>. Anatomy of S. fruticosa under different salinity level<br />

Table 19. C δ value of Suaeda fruticosa subjected to different salinity levels<br />

C<br />

δ value<br />

control <strong>12</strong> ds m -1 18 ds m -1 24 ds m -1 30 ds m -1 36 ds m -1<br />

Suaeda fruticosa 14.543 16.721 15.<strong>12</strong>5 14.659 13.876 8.698<br />

Phenomics of moisture deficit and low<br />

temperature stress tolerance in rice<br />

Fifteen days old seedlings of 525 germplasm accessions<br />

were exposed to seedling stage/ early vegetative<br />

stage moisture stress under field condition with four<br />

blocks and five checks (tolerant and susceptible). Soil<br />

moisture content was 2.38 to 11.20% and soil moisture<br />

tension was 50- 60 kPa during the stress period under<br />

15 cm soil depth. Leaf death score was recorded following<br />

IRRI SES ‘0’ to ‘9’scale. Out of 525 entries, 37 entries<br />

were observed to be tolerant with drought and recovery<br />

score SES ‘1’, 33 as moderately tolerant with SES score<br />

‘3’, 108 relatively less tolerant with SES score ‘5’, 236<br />

with SES score ‘7’ and 111 as susceptible with SES<br />

score ‘9’. The tolerant checks (Vandana, Vanaprabha,<br />

Salumpikit and CR143-2-2) performed better with SES<br />

score ranging between ‘1-5’, whereas susceptible check<br />

(IR20) was observed with SES score in the range of ‘7-9’.<br />

recorded when most of the entries showed leaf rolling<br />

symptom and the score was taken following IRRI SES<br />

‘0’ to ‘9’scale. Out of 525 entries, 75 entries were observed<br />

to be highly tolerant with drought and recovery<br />

score SES ‘1’ followed by 188 as moderately tolerant<br />

with SES score ‘3’, 92 relatively less tolerant with SES<br />

score ‘5’, 134 with SES score ‘7’ and 36 as susceptible<br />

with SES score ‘9’. The tolerant checks performed better<br />

with SES score ranging between ‘1-5’, whereas susceptible<br />

check (IR-20) was observed with SES score in<br />

the range of ‘7-9’.<br />

Bioprospecting and Allele Mining<br />

Against Abiotic Stresses<br />

Phenotyping under salt stress and genotyping<br />

using QTL linked markers<br />

A total of 825 lines were sown at CRRI and thirty<br />

day old seedlings were transplanted in the field at<br />

50 CRRI ANNUAL REPORT 2011-<strong>12</strong>

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