Central Rice Research Institute Annual report...2011-12

Development and evaluation of superior plant
types to raise yield ceilings in uplands
F 2
population of 28 crosses was raised during wet
season 2011 under transplanted condition at CRRI
Regional Station, Hazaribag. Based on the performance
of the crosses, a total of 704 single plant selections were
made from 19 crosses. All these selections were generation
advanced at Cuttack during 2012 dry season. Selection
was not done in F 3
and progenies were harvested
as bulks. Selection will be made in F 4
generation that
will be grown at Hazaribag under direct seeded condition
during 2012 wet season.
F 2
population under moderate stress during wet season
2011 at CRRI Regional Station, Hazaribag
84-2-2-2-1(8.03 t ha -1 ), CR3300-3-1-1(7.8 t ha -1 ) and IR
72158-68-6-3-3-1(7.39 t ha -1 ). Apart from grain yield,
CR3299-11-1-3 with long panicle, IR 73930-41-5-3-1-5-
2 and IR71700-247-5-3-2-1-22-1with long top three
leaves; IR71701-28-1-4 -1 and IR77700-84-2-2-2 with
higher leaf breadth; IR77298-5-6 and IR78585-98-2-2-1
with compact panicle with good grain filling; and
IR73896-51-2-1-3-2 with overall superior performance.
Seven elite cultures viz., CR3299-11-1-1-1, IR72158-
26-3-CR3624-3-1-1, CR3300-1-2-1-1, CR3299-2-1-1-1,
IR77700-84-2-2-2-CR3623-2-2-1, CR3299-10-1-3-1,
CR3624-4 were nominated for AICRIP trials and
CR2996-1-14-29-3-1(IET-22731) was promoted to
AVT 1.
Development and evaluation of superior plant
types for unfavourable ecosystems
Seventy two F 7
generation superior single plant progenies
of forty five inter sub-specific derivatives were
grown along with three check varieties for observational
yield evaluation. In the inter sub-specific crossing
programme, three high yielding varieties of lowland
were used as female parents and fifteen tropical japonica
lines possessing traits like more spikelets panicle -1 ,
thick upright and broad leaf, long panicle and high
seed test weight used as male parents for generating
forty five F 1
hybrids. CR2683-15-5-3-1-1, CR2683-7-2-3-
1-1, CR3608-1-2-1-1-1, CR2690-2-2-1-1-1 and CR3611-
1-1-2-1-1 were observed to be very promising in the observational
yield evaluation trial. CR2683-15-5-3-1-1 is
in the final year of testing in national trial under rainfed
shallow lowlands.
DNA Fingerprinting of Rice Varieties,
Hybrids and other Elite Germplasm and
Development of Database
DNA fingerprinting and evaluation of genetic
relatedness in aromatic landraces
A total of 56 (nine aromatic varieties and 47
landraces) including five non-aromatic landraces were
characterized using SSR and ISSR markers for fingerprinting
and assessing the genetic variation among
them. A pre-screening of 32 SSR primers pairs was performed
out of which only 29 SSR primers produced a
total of 68 bands of which 35 bands (51.47%) were polymorphic
in nature. The PIC values varied from 0.0645
for primer RM431 to 0.919 for primer RM144 with a
mean of 0.618. Similarly, a total of 35 ISSR primers were
employed out of which 14 primers could produce clear
and reproducible bands. A total of 84 bands were amplified,
of which 42 and six were polymorphic and
unique respectively.
The four microsatellite primer pairs such as RM152,
RM312, RM433 and RM144 were able to identify and
discriminate the aromatic landraces Sunabasmati,
Lajkulibad, Kalajeera and one non-aromatic landraces
Premanjalli. Likewise the six ISSR primers could also
identify and differentiate six aromatic rice landraces
viz., Mohanbhog, Kalkati, Kapurkanti, Bindli,
Bishnubhog and Jaigundi collected from different districts
of Odisha along with one non-aromatic rice
Gotikiamali and another CRRI released aromatic rice
variety Nua Chinikamini.
Dendrogram constructed from combined SSR and
ISSR data segregated two landraces (Ganjeijata and
Badsabhog) and Nua Chinikamini from rest of the released
aromatic rice varieties, landraces and non-aromatic
landraces sharing a node at 36% level of similar-
32 CRRI ANNUAL REPORT 2011-12