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Central Rice Research Institute Annual report...2011-12

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Developing IPM Technologies for Different <strong>Rice</strong> Ecologies<br />

Table 41. Fungitoxicity of aqueous extract of Aegle marmelos, mother extract (AME)/in combination with formulating<br />

agent FA(B + ) on conidial germination of Pyricularia grisea<br />

Treatment<br />

(FA/AME) Concentration (%)<br />

10% 1% 0.1% 0.01% 0.001% 0.0001% Control(FA)<br />

Conidial germination (%)<br />

10% 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) a<br />

1% 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a<br />

0.1% 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a<br />

0.01% 2 (8.13) a 2 (8.13) a 30 (33.21) bg 55 (47.57) bg 65 (53.73) b 70 (56.79) b 80 (63.44) b<br />

0.001% 30 (33.21) bg 70 (56.79) c 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87)<br />

0.0001% 50 (45.00) c 95 (77.08) c 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87)<br />

Control(AME) 2 (8.13) * 2 (8.13) a 50 (45.00) bg 70 (56.79) C 98 (81.87) 98 (81.87)<br />

Control(sterilized d.w.) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87)<br />

C.D. 5%-0.21<br />

Data in parentheses represents angular values; Complete inhibition is represented as 2%; *=complete bursting; a=<br />

ungerminated conidia; b= reduced germ tube; g= granulated germ tube; c= longer germ tube; AME=aqueous<br />

mother extract; FA= formulating agent<br />

b. Distortion in P. grisea coinidia<br />

Normal Ungerminated Reduced &<br />

germination coinidia granulated<br />

germ tube<br />

c. Distortion in H. oryzae coinidia<br />

Normal<br />

germination<br />

Reduced germ<br />

tube<br />

Completely inhibited<br />

conidia<br />

Excessively longer<br />

and highly branched<br />

germ tube<br />

Fungitoxic spectrum of twelve new plants viz.,<br />

Thevetia peruviana L., Mollugo pentaphylla Linn., Moringa<br />

oleifera Lam.,Vitex negundo L., Citrus limon L., Murrya<br />

koenigii L., Callistemon viminalis Gaertn., Phyllanthus<br />

emblica L. was tested in ethanolic extract (1:4 w/v) and<br />

Euphorbia hirta L., Ageratum conyzoides L., Physalis<br />

minima L., Phyllanthus niruri L. was tested as a series of<br />

solutions ranging from 0.001 to 100% concentration in<br />

aqueous extract (1:4 w/v) against the primary inoculum,<br />

conidia of P. grisea, H. oryzae, C. lunata and Fusarium<br />

moniliforme, respectively. All the treatments drastically<br />

reduced conidial germination at and above 0.1% concentration<br />

and from 1% concentration in except C. limon<br />

and C. viminalis, ethanolic extract which displayed<br />

higher reduction in P. grisea. Critical fungi toxic pattern<br />

such as granulation, reduction/elongation, thickening,<br />

coiling, bulbular structure at the base of germ<br />

tube and highly branched germ tube was observed in<br />

treated conidia compared to normal germination in<br />

control.<br />

Identification of effective botanicals and their<br />

evaluation against blast<br />

Six plant extracts of commonly occurring grasses<br />

and weeds (Australian Grass, Cynodon dactylan with<br />

and without roots, Parthenium, Lantana and Ipomea) along<br />

with control as chemical check were evaluated under<br />

field condition against brown spot of rice in upland.<br />

Preliminary study on the efficacy of the fresh plant extracts<br />

revealed that they have potential to control brown<br />

CRRI ANNUAL REPORT 2011-<strong>12</strong><br />

89

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