Central Rice Research Institute Annual report...2011-12
Central Rice Research Institute Annual report...2011-12
Central Rice Research Institute Annual report...2011-12
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Developing IPM Technologies for Different <strong>Rice</strong> Ecologies<br />
Table 41. Fungitoxicity of aqueous extract of Aegle marmelos, mother extract (AME)/in combination with formulating<br />
agent FA(B + ) on conidial germination of Pyricularia grisea<br />
Treatment<br />
(FA/AME) Concentration (%)<br />
10% 1% 0.1% 0.01% 0.001% 0.0001% Control(FA)<br />
Conidial germination (%)<br />
10% 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) * 2 (8.13) a<br />
1% 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a<br />
0.1% 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a 2 (8.13) a<br />
0.01% 2 (8.13) a 2 (8.13) a 30 (33.21) bg 55 (47.57) bg 65 (53.73) b 70 (56.79) b 80 (63.44) b<br />
0.001% 30 (33.21) bg 70 (56.79) c 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87)<br />
0.0001% 50 (45.00) c 95 (77.08) c 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87)<br />
Control(AME) 2 (8.13) * 2 (8.13) a 50 (45.00) bg 70 (56.79) C 98 (81.87) 98 (81.87)<br />
Control(sterilized d.w.) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87) 98 (81.87)<br />
C.D. 5%-0.21<br />
Data in parentheses represents angular values; Complete inhibition is represented as 2%; *=complete bursting; a=<br />
ungerminated conidia; b= reduced germ tube; g= granulated germ tube; c= longer germ tube; AME=aqueous<br />
mother extract; FA= formulating agent<br />
b. Distortion in P. grisea coinidia<br />
Normal Ungerminated Reduced &<br />
germination coinidia granulated<br />
germ tube<br />
c. Distortion in H. oryzae coinidia<br />
Normal<br />
germination<br />
Reduced germ<br />
tube<br />
Completely inhibited<br />
conidia<br />
Excessively longer<br />
and highly branched<br />
germ tube<br />
Fungitoxic spectrum of twelve new plants viz.,<br />
Thevetia peruviana L., Mollugo pentaphylla Linn., Moringa<br />
oleifera Lam.,Vitex negundo L., Citrus limon L., Murrya<br />
koenigii L., Callistemon viminalis Gaertn., Phyllanthus<br />
emblica L. was tested in ethanolic extract (1:4 w/v) and<br />
Euphorbia hirta L., Ageratum conyzoides L., Physalis<br />
minima L., Phyllanthus niruri L. was tested as a series of<br />
solutions ranging from 0.001 to 100% concentration in<br />
aqueous extract (1:4 w/v) against the primary inoculum,<br />
conidia of P. grisea, H. oryzae, C. lunata and Fusarium<br />
moniliforme, respectively. All the treatments drastically<br />
reduced conidial germination at and above 0.1% concentration<br />
and from 1% concentration in except C. limon<br />
and C. viminalis, ethanolic extract which displayed<br />
higher reduction in P. grisea. Critical fungi toxic pattern<br />
such as granulation, reduction/elongation, thickening,<br />
coiling, bulbular structure at the base of germ<br />
tube and highly branched germ tube was observed in<br />
treated conidia compared to normal germination in<br />
control.<br />
Identification of effective botanicals and their<br />
evaluation against blast<br />
Six plant extracts of commonly occurring grasses<br />
and weeds (Australian Grass, Cynodon dactylan with<br />
and without roots, Parthenium, Lantana and Ipomea) along<br />
with control as chemical check were evaluated under<br />
field condition against brown spot of rice in upland.<br />
Preliminary study on the efficacy of the fresh plant extracts<br />
revealed that they have potential to control brown<br />
CRRI ANNUAL REPORT 2011-<strong>12</strong><br />
89