rok 2007 - Fakulta chemickej a potravinárskej technológie

Cultivation media composition as well as the process parameters will be optimized. To minimize the toxic effects
of the product towards the culture, the mechanism and characteristics of in-situ product extraction during the
fermentation process will be used and studied. A suitable organic phase will be selected and tested for in-situ
extraction of the product. A continuous product separation system will be constructed and integrated into the
system bioreactor - in-situ extraction - separation. Finally. the integrated process for 2-phenylethanol will be
designed and optimized in lab- and pilot-scale experiments. Optimal parameters of the batch and continuous
processes will be proposed and described. Process data should be used for design of the industrial scale process
for production of 2-phenylethanol.
Project duration: from 01. 03.06 to 31.12. 09
N. APVV-51-033205, Genetically modified microorganisms as whole cells biocatalysts of
enantioselective biooxydations for new immobilized biotechnologies (Vladimír Štefuca)
The project is aimed at the development of immobilized cell biocatalysts for oxidation of prochiral compounds
through the Baeyer-Villiger reaction mechanism. The basic tasks of the Faculty involved are: 1. Development of
an optimum biocatalyst particle using experimental and mathematical modeling involving kinetic and mass
transfer phenomena; 2. Design and scale up of bioreactors for oxidative biotransformations of chosen
compounds.
Project duration: from 2006 to 2008
O. APVV project LPP-0234-06, Chromatographic separation of fruktooligosaccharides (Milan
Polakovič)
This project is carried within a programme aimed at the stimulation and support of excellent PhD. supervisors.
The content of the project is associated with the education of a PhD. student having the topic of the PhD. study
identical with the title of the project.
Project duration: from 13.11. 2006 to 31.11.2009
P. VEGA project 1/3582/06, Mechanisms and kinetics of enzyme inactivation (Vladimír Štefuca)
Enzyme inactivation is a phenomenon, which has unfavorable impact in various areas, such as biocatalytic
productions, food processing or application of diagnostic tests. A way to the increase of process stability of
enzymes can be a better understanding of their inactivation mechanisms. The primary goal of this project is the
investigation of the mechanism of heat and pH inactivation of enzymes in real media, in which the effect of
stabilization of enzymes by components of the solution is manifested. The method of identification of inactivation
mechanisms is based on a consequent connection of the design of experiments, its performance under a broad
range of conditions and evaluation of the kinetics by mathematical modeling. In special cases, the investigation of
the mechanism of inactivation will be based on a combined treatment of kinetic data of the loss of enzyme activity
and quantities characterizing structural changes, as intensity of laser light scattering or molecular weight
distribution obtained by electrophoresis or chromatography. The methods of the investigation of mechanisms are
directly related to the elaboration of a procedure for quick determination of process stability of an immobilized
biocatalyst in a differential fixed bed reactor.
Project duration: from 01.01. 2006 to 31.12.2008
Q. VEGA project 1/3565/06, Engineering aspects of chromatographic separation of proteins
and saccharides (Milan Polakovič)
The project deals with the investigation of engineering aspects of chromatographic separation of a mixture of
proteins and saccharides, which are subject of practical interest. Particularly, the purification of IgG,
fructosyltransferase (FTase) and fructooligosaccharides (FOS’s) has been studied. A problem of general interest
is the investigation of the accessibility of pores by batch and column size-exclusion techniques. The aim is the
assessment of advantages, disadvantages and limitations of both techniques and the possibility of interpretation
of basic data in the form of pore size distribution. A further part of the project includes investigation of equilibrium
and kinetics of adsorption of proteins on affinity and ion-exchange adsorbents by a batch method. The
investigation of multi-component adsorption equilibria of saccharides on ion-exchangers will be one of the
important parts of the project for their further utilization for modeling and designing separation in a simulated
moving bed. In the area of separation of enzymes the project will be aimed at characterizing equilibria in various
concentrations of salts, which can be employed in mathematical description of separation using salt gradient. The
last area of interest will concern the investigation of kinetics of affinity sorption of FTase on an adsorbent with a
bound lectin ligand.
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