Vol. 16—1962 - NorthEastern Weed Science Society

non-polar solvents. Table 5 shows that an inhibition extracted with chloro#o~
can be separated from the chloroform by shaking with water in a separatory 'funnel.
Similar results were obtained with benzene.
Table S. Removal of activity from chloroform with
water in a separatory funnel •
._-._._---_..-.-..... _.-..---------------_.-.-.--
Percent
geradnation
Percent
inhibition
---_._.-._-_---.' -.- - _-----_.
Extracted
Chloroform
Water Extract
80
30
24
70'
235
Whenone g. dry meal was asbed at 625OC. for 4 hours and 20 ml. water
added to the ash to makeup a test solution, alfalfa seedlings grew as well: as
,controls (Table 6). The ash solution was basic (pH 12.2). HN03was added"to
bring the solution to pH 7. However, seedlings grew well in both solutio~s.
Growth in the basic solution is explained by spcretion of organic acids from
the alfalfa s4edlings as the pH in the basic test bad come down to near pH 7
at the end of 4 days seedling growth.
Table 6
The effect of ash on inhibltion of'
alfalfa seedlings.
Dialysis
-----------------~_!~~~~~--_:~~~~~~~~~-----
Control 37 00
Ash (No neutral~aatten
treatment)' 42 -13
Ash (Neutralized
with HN0 3) 39 - 5
.-.~--~._-.--.---...._._..----_..-_.
__.._---
Autoclaved extract was placed in cellophane dtalycer tubing and the tubing
inserted in a cylinder. Water in the cylinder was changed about 10 times during
three days treatment. All material outside the cylinder and the heavy concentrate
which did not pass through the casing were concentrated and diluted for
asaay. Table 7 shows most of the activity passed through the cellophane tubing.